Molecular Analysis of 'Candidatus Phytoplasma trifolii' and 'Candidatus Phytoplasma solani' Associated with Phytoplasma Diseases of Tomato (PDT) in Turkey

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dc.authorwosidUsta, Mustafa/CAG-7521-2022
dc.authorwosidSipahioglu, Hikmet Murat/AAA-6085-2020
dc.contributor.authorUsta, Mustafa
dc.contributor.authorGuller, Abdullah
dc.contributor.authorSipahioglu, Hikmet Murat
dc.date.accessioned2024-08-04T20:45:21Z
dc.date.available2024-08-04T20:45:21Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractTomato plants displaying severe fruit deformation, flower sterility, aerial rooting, purplish leaves and leaf rolling were observed in tomato fields at Van province (Turkey). Samples were collected, and total DNA was extracted from symptomatic and asymptomatic plants. Nested polymerase chain reaction (nested-PCR) assays were performed to amplify 16S rDNA sequences for molecular detection using universal primer pairs. Out of 100 tested tomato samples, 11% of tomato samples yielded a DNA fragment of 1.25 kb. Amplified PCR products were then cloned into pGEM T-Easy vector and sequenced using new generation DNA sequencing (NGS) system. The virtual restriction fragment length polymorphism (RFLP) analysis of 16S rDNA sequences and molecular detections were allowed to characterize possible phytoplasmas associated with diseased plants. Our results revealed the presence of two Phytoplasma species belonging to two different ribosomal groups; 'Candidatus Phytoplasma trifolii' (16Sr VI-A group) (Acces no. MF564268, MG732925) and 'Candidatus Phytoplasma solani' (16SrXII-A group) (Acces no. KY579358, MF576263). Despite a high variation in their similarity coefficient of'Ca. P. solani' VTS2 (0.91) and 'Ca. P. trifolii' VTT1 (0.88) isolates, the infected tomato plants generally displayed similar disease symptoms during field observations. Due to its commercial interest, co-existing of these phytoplasmas in tomato fields is of great phytosanitary significance not only for tomato plants but also for other crops such as vegetables, ornamentals and field crops. With this study, 'Ca. P. trifolii' associated with phytoplasma diseases of tomato (PDT) has been reported for the first time in tomato in Turkey. (C) 2018 Friends Science Publishersen_US
dc.description.sponsorshipYuzuncu Yil University [FHD-2016-5205]en_US
dc.description.sponsorshipThis research was supported by the Research Fund of Yuzuncu Yil University (Project no FHD-2016-5205).en_US
dc.identifier.doi10.17957/IJAB/15.0721
dc.identifier.endpage1996en_US
dc.identifier.issn1560-8530
dc.identifier.issn1814-9596
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-85050718515en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1991en_US
dc.identifier.urihttps://doi.org/10.17957/IJAB/15.0721
dc.identifier.urihttps://hdl.handle.net/11616/98403
dc.identifier.volume20en_US
dc.identifier.wosWOS:000440740600009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherFriends Science Publen_US
dc.relation.ispartofInternational Journal of Agriculture and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCandidatus Phytoplasma trifolii'en_US
dc.subject'Candidatus Phytoplasma solani'en_US
dc.subjectMolecular analysisen_US
dc.subjectSolanum lycopersicumen_US
dc.titleMolecular Analysis of 'Candidatus Phytoplasma trifolii' and 'Candidatus Phytoplasma solani' Associated with Phytoplasma Diseases of Tomato (PDT) in Turkeyen_US
dc.typeArticleen_US

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