Bioassay-guided isolation and antiproliferative efficacy of extract loaded in chitosan nanoparticles and LC-QTOF-MS/MS analysis of Achillea magnifica

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dc.authoridtaskin, turgut/0000-0001-8475-6478
dc.authoridARABACI, Turan/0000-0003-3869-4557
dc.authoridDoğan, Murat/0000-0003-2794-0177
dc.authorwosidtaskin, turgut/AAE-4680-2020
dc.authorwosidARABACI, Turan/AAQ-3885-2020
dc.authorwosidDoğan, Murat/ABF-6037-2020
dc.contributor.authorTaskin, Turgut
dc.contributor.authorDogan, Murat
dc.contributor.authorArabaci, Turan
dc.date.accessioned2024-08-04T20:48:47Z
dc.date.available2024-08-04T20:48:47Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study investigated the antioxidant, anti-urease, anticholinesterase, and antiproliferative activities of different extracts of A. magnifica. The active compounds were isolated from chloroform extract, which has the strongest antiproliferative and antioxidant activities. The structures of the isolated compounds (elenolic acid, luteolin, eupatilin) were examined using spectroscopic techniques (NMR, UV, HR-MS). The antioxidant and antiproliferative effects of isolated compounds showed lower biological activity than those of the chloroform extract. Therefore, chitosan nanoparticles were prepared with this extract and characterized. The cytotoxic activities of the chitosan nanoparticles were examined, and they were found to have a strong antiproliferative activity similar to that of the chloroform extract. The ethanol extract had strong anti-urease and anticholinesterase activity compared with other extracts. Therefore, phenolic compounds that might be responsible for the biological activity were analysed by LC-QTOF-MS/MS. The ferulic acid derivative, apigenin 6 C pentoside 8 C hexoside, apigenin 6,8-di C-hexoside, vitexin, and diosmetin 8-C-glucoside (orientin 4'-methyl ether) compounds from this plant were analysed for the first time. The results showed that the chloroform-loaded chitosan nanoparticles had a strong antiproliferative activity in breast cancer cell lines (MCF-7, MDA-MB-231) and no toxic effect on normal cell lines (L-929), which suggested that this extract might be a potential candidate as a herbal medicine that can be investigated and evaluated in future cancer studies. Additionally, the potential use of ethanol extract as an anti-urease and anticholinesterase agent was revealed. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.sajb.2020.08.002
dc.identifier.endpage244en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85089483124en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage236en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2020.08.002
dc.identifier.urihttps://hdl.handle.net/11616/99460
dc.identifier.volume133en_US
dc.identifier.wosWOS:000571185800011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSouth African Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectA. magnificaen_US
dc.subjectChitosan nanoparticlesen_US
dc.subjectBiological activityen_US
dc.subjectLC-QTOF-MS/MSen_US
dc.titleBioassay-guided isolation and antiproliferative efficacy of extract loaded in chitosan nanoparticles and LC-QTOF-MS/MS analysis of Achillea magnificaen_US
dc.typeArticleen_US

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