Evaluation of Caco-2 cell permeability of ritonavir nanosuspensions

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dc.authoridKarakucuk, Alptug/0000-0002-9061-2427
dc.authoridOzturk, Naile/0000-0002-7617-8433
dc.authorwosidKarakucuk, Alptug/AAI-2354-2019
dc.authorwosidCELEBİ, Nevin/AAL-6931-2021
dc.authorwosidKarakucuk, Alptug/JMQ-2136-2023
dc.authorwosidOzturk, Naile/F-3650-2017
dc.contributor.authorKarakucuk, Alptug
dc.contributor.authorOzturk, Naile
dc.contributor.authorCelebi, Nevin
dc.date.accessioned2024-08-04T20:10:32Z
dc.date.available2024-08-04T20:10:32Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground and Aims: Poor aqueous solubility limits drug absorption through intestinal mucosa. Nanosuspensions with nanometer range particle size provides enhanced aqueous solubility and hence permeability. The objective of this study was to investigate the cytotoxicity and in vitro cell permeability through human adenocarcinoma (Caco-2) cells of ritonavir (RTV) nanosuspensions. Methods: The Microfluidization method was used to prepare nanosuspensions. Particle size (PS), polydispersity index (PI) and zeta potential (ZP) values were measured as characterization. MTT test was applied to evaluate the cytotoxic effect. Caco-2 cell lines were used for transport studies with RTV coarse powder, physical mixtures and nanosuspension. Results: Approximately 600 nm PS, 0.4 PDI and 22 mV ZP values were observed for nanosuspensions. The sample groups showed no cytotoxicity on the cell lines in any RTV concentration. However, significant cytotoxic effect was determined in groups with high amounts of sodium dodecyl sulfate. The transported RN in nanosuspension formulation enhanced by 5.3-fold and 1.5-fold in comparison with RTV coarse powder and physical mixture, respectively. Rate of the transportation and also the amount of the transported RTV were improved with nanosuspension formulation. Conclusion: Particle size reduction of RTV into nanometer size and preparing nanosuspension system was found effective to obtain enhanced cell permeability.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [113S842]; (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by a grant from The Scientific and Technological Research Council of Turkey. (Project No: 113S842, TUBITAK)en_US
dc.identifier.doi10.26650/IstanbulJPharm.2020.0028
dc.identifier.endpage255en_US
dc.identifier.issn2587-2087
dc.identifier.issue3en_US
dc.identifier.startpage251en_US
dc.identifier.trdizinid420861en_US
dc.identifier.urihttps://doi.org/10.26650/IstanbulJPharm.2020.0028
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/420861
dc.identifier.urihttps://hdl.handle.net/11616/92849
dc.identifier.volume50en_US
dc.identifier.wosWOS:000605357000014en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherIstanbul Univ, Fac Pharmacyen_US
dc.relation.ispartofIstanbul Journal of Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRitonaviren_US
dc.subjectnanosuspensionen_US
dc.subjectCaco-2en_US
dc.subjectpermeabilityen_US
dc.titleEvaluation of Caco-2 cell permeability of ritonavir nanosuspensionsen_US
dc.typeArticleen_US

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