Effects of melatonin on both testicular regeneration and recovery of spermatogenesis in busulfan-treated rats

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dc.authoridEşrefoğlu, Mukaddes/0000-0003-3380-1480
dc.authoridTaslidere, Elif/0000-0003-1723-2556
dc.authorwosidTok, Olgu Enis/HGE-3451-2022
dc.authorwosidEşrefoğlu, Mukaddes/JWA-4590-2024
dc.authorwosidTaslidere, Elif/ABI-8046-2020
dc.contributor.authorTaslidere, E.
dc.contributor.authorEsrefoglu, M.
dc.contributor.authorTok, O. E.
dc.contributor.authorTaslidere, B.
dc.contributor.authorBulut, H.
dc.date.accessioned2024-08-04T20:53:21Z
dc.date.available2024-08-04T20:53:21Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractTesticular damage is one of the most hazardous effects of chemotherapy as it is frequently associated with oligozoospermia and azoospermia. This study aimed at evaluating the protective effect of melatonin in a rat model of busulfan-induced testicular injury. Rats were divided into four groups: control, melatonin, busulfan, busulfan plus melatonin. After 15 days, the semen was collected from the epididymis and testes were assessed. Sperm removed from cauda epididymis and analyzed for sperm count and viability. Testis tissues were also removed, fixed in formalin and were embedded in paraffin. Sections of testis tissue were stained with hematoxylin-eosin for histological examination and prepared for TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay to detect apoptosis and PCNA (proliferating cell nuclear antigenassay) to detect proliferation cells. Serum and testes supernatants were separated to detect testosteron level and oxidative stress parameters. In histological examination, degenerative changes in seminiferous tubules were observed in the experimental groups. In biochemical examination, the total oxidant status (TOS) levels in Busulfan group were significantly higher than in the control group while the total antioxidant status (TAS) levels of all the groups were similar. In conclusion, the beneficial properties of melatonin treatment by its potent anti-oxidants may reduce adverse effects of chemotherapy in the reproductive system in a rodent system.en_US
dc.identifier.doi10.1590/s2175-97902022e20945
dc.identifier.issn1984-8250
dc.identifier.issn2175-9790
dc.identifier.scopus2-s2.0-85145891039en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1590/s2175-97902022e20945
dc.identifier.urihttps://hdl.handle.net/11616/101103
dc.identifier.volume58en_US
dc.identifier.wosWOS:000917303400001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Sao Paulo, Conjunto Quimicasen_US
dc.relation.ispartofBrazilian Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBusulfanen_US
dc.subjectMelatoninen_US
dc.subjectSpermatogenesisen_US
dc.subjectTestisen_US
dc.subjectRaten_US
dc.titleEffects of melatonin on both testicular regeneration and recovery of spermatogenesis in busulfan-treated ratsen_US
dc.typeArticleen_US

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