Identification of respiratory chain gene mutations that shorten replicative life span in yeast
Küçük Resim Yok
Dosyalar
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Experimental Gerontology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aging is the progressive accumulation of alterations in cells that elevates the risk of death. The mitochondrial
theory of aging postulates that free radicals produced by the mitochondrial respiratory system contribute to
the aging process. However, the roles of individual electron transfer chain (ETC) components in cellular aging
have not been elucidated. In this study, we analyzed the replicative life span of 73 yeast deletion mutants
lacking the genes of the mitochondrial electron transfer chain system, and found that nine of these mutants
(Δnde1, Δtcm62, Δrip1, Δcyt1, Δqrc8, Δpet117, Δcox11, Δatp11, Δfmc1) had significantly shorter life spans.
These mutants had lower rates of respiration and were slightly sensitive to exogenous administration of hydrogen
peroxide. However, only two of them, Δnde1 and Δfmc1, produced higher amounts of intrinsic superoxide
radicals in the presence of glucose compared to that of wild type cells. Interestingly, there were no
significant alterations in the mitochondrial membrane potentials of these mutants. We speculate that the
shorter life spans of ETC mutants result from multiple mechanisms including the low respiration rate and
low energy production rather than just a ROS-dependent path.
Açıklama
Experimental Gerontology 47 (2012) 149–153
Anahtar Kelimeler
Mitochondria, Electron transport chain (ETC), Reactive oxygen species (ROS), Aging, Replicative life span, Chronological aging, Longevity, Yeast
Kaynak
Experimental Gerontology
WoS Q Değeri
Scopus Q Değeri
Cilt
47
Sayı
0
Künye
Elise, H., DEMİR, A. B., & KOÇ, A. (2012). Identification of respiratory chain gene mutations that shorten replicative life span in yeast. Experimental Gerontology, 47(2), 149–153.
