Identification of respiratory chain gene mutations that shorten replicative life span in yeast

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dc.authorid190238en_US
dc.authorid110769en_US
dc.contributor.authorHacıoğlu, Elise
dc.contributor.authorDemir, Ayşe Banu
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2017-06-10T08:19:34Z
dc.date.available2017-06-10T08:19:34Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.descriptionExperimental Gerontology 47 (2012) 149–153en_US
dc.description.abstractAging is the progressive accumulation of alterations in cells that elevates the risk of death. The mitochondrial theory of aging postulates that free radicals produced by the mitochondrial respiratory system contribute to the aging process. However, the roles of individual electron transfer chain (ETC) components in cellular aging have not been elucidated. In this study, we analyzed the replicative life span of 73 yeast deletion mutants lacking the genes of the mitochondrial electron transfer chain system, and found that nine of these mutants (Δnde1, Δtcm62, Δrip1, Δcyt1, Δqrc8, Δpet117, Δcox11, Δatp11, Δfmc1) had significantly shorter life spans. These mutants had lower rates of respiration and were slightly sensitive to exogenous administration of hydrogen peroxide. However, only two of them, Δnde1 and Δfmc1, produced higher amounts of intrinsic superoxide radicals in the presence of glucose compared to that of wild type cells. Interestingly, there were no significant alterations in the mitochondrial membrane potentials of these mutants. We speculate that the shorter life spans of ETC mutants result from multiple mechanisms including the low respiration rate and low energy production rather than just a ROS-dependent path.en_US
dc.identifier.citationElise, H., DEMİR, A. B., & KOÇ, A. (2012). Identification of respiratory chain gene mutations that shorten replicative life span in yeast. Experimental Gerontology, 47(2), 149–153.en_US
dc.identifier.doi10.1016/j.exger.2011.11.009en_US
dc.identifier.endpage153en_US
dc.identifier.issue0en_US
dc.identifier.startpage149en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S0531556511003184
dc.identifier.urihttps://hdl.handle.net/11616/7076
dc.identifier.volume47en_US
dc.language.isoenen_US
dc.publisherExperimental Gerontologyen_US
dc.relation.ispartofExperimental Gerontologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMitochondriaen_US
dc.subjectElectron transport chain (ETC)en_US
dc.subjectReactive oxygen species (ROS)en_US
dc.subjectAgingen_US
dc.subjectReplicative life spanen_US
dc.subjectChronological agingen_US
dc.subjectLongevityen_US
dc.subjectYeasten_US
dc.titleIdentification of respiratory chain gene mutations that shorten replicative life span in yeasten_US
dc.typeArticleen_US

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