Developmental, toxicological effects and recovery patterns in Xenopus laevis after exposure to penconazole-based fungicide during the metamorphosis process

Basit Öğe Kaydı
dc.authoridturhan, duygu ozhan/0000-0002-7111-4289
dc.authoridGungordu, Abbas/0000-0001-7770-0660
dc.authorwosidturhan, duygu ozhan/AAA-3247-2021
dc.authorwosidGungordu, Abbas/A-3009-2015
dc.contributor.authorTurhan, Duygu Ozhan
dc.contributor.authorGungordu, Abbas
dc.date.accessioned2024-08-04T20:52:03Z
dc.date.available2024-08-04T20:52:03Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractFungicides are a group of chemicals causing pollution of freshwater ecosystems due to their widespread use in agriculture. However, their endocrine disrupting effects are less studied than herbicides and insecticides. The aim of this study was to evaluate the developmental and toxicological effects and recovery patterns of penconazolebased fungicide (PBF) during Xenopus laevis metamorphosis. For this purpose, firstly, the 96 h median lethal (LC50) and effective (EC50) concentrations and minimum concentration to inhibit growth (MCIG) values of PBF were estimated for X. laevis as 4.97, 3.55 and 2.31 mg/L respectively, using Frog Embryo Teratogenesis AssayXenopus (FETAX) on Nieuwkoop-Faber (NF) stage 8 embryos. FETAX results showed PBF formulation was slightly teratogenic with a 1.4 teratogenic index; most recorded malformations were gut, abdominal edema, and tail curvature. The Subacute Amphibian Metamorphosis Assay (AMA) was modified based on acute FETAX results, and used to evaluate toxic effects and recovery patterns of relatively low PBF concentrations on metamorphosis using morphological and biochemical markers. NF Stage 51 tadpoles were exposed to two separate groups of each concentration for seven days in the AMA. Secondly, tadpoles of one group of each concentration continued to be exposed to PBF for the next 7 and 14 days while the other group was kept in a pesticide-free environment (depuration/recovery). Various morphological and biochemical markers were measured homogenate samples of tadpoles from exposure and recovery groups. Continuous exposure to relatively low PBF concentrations caused oxidative stress, toxic, and endocrine disrupting effects in the AMA, leading us to conclude that it has negative effects on frog health and development during the recovery period when PBF exposure is terminated. The glutathione S-transferase, glutathione reductase, catalase, carboxylesterase, and acetylcholinesterase activities were higher than the control group transferred to pesticide-free media for 14 days after the 7 days exposure and indicate persistent PBF impact.en_US
dc.identifier.doi10.1016/j.chemosphere.2022.135302
dc.identifier.issn0045-6535
dc.identifier.issn1879-1298
dc.identifier.pmid35697111en_US
dc.identifier.scopus2-s2.0-85131781432en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2022.135302
dc.identifier.urihttps://hdl.handle.net/11616/100712
dc.identifier.volume303en_US
dc.identifier.wosWOS:000813230700008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofChemosphereen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPenconazoleen_US
dc.subjectXenopus laevisen_US
dc.subjectFETAXen_US
dc.subjectAMAen_US
dc.subjectBiochemical markersen_US
dc.subjectEndocrine disruptionen_US
dc.titleDevelopmental, toxicological effects and recovery patterns in Xenopus laevis after exposure to penconazole-based fungicide during the metamorphosis processen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu