Investigation for the presence of bacteria and antimicrobial resistance genes in sea snails (Rapana venosa)

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dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridTanriverdi, Elif Seren/0000-0002-0449-0356
dc.authoridZigo, František/0000-0002-2791-166X
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidTanriverdi, Elif Seren/ABE-4472-2021
dc.authorwosidZigo, František/AEO-3938-2022
dc.contributor.authorOzbey, Gokben
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorBasusta, Asiye
dc.contributor.authorLakshmanappa, Yashvanth Shaan
dc.contributor.authorOtlu, Baris
dc.contributor.authorZigo, Frantisek
dc.date.accessioned2024-08-04T20:54:28Z
dc.date.available2024-08-04T20:54:28Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIntroduction and Objective. The aims of this study were to search for the presence of bacteria in sea snails (Rapana venosa) by using culturomics and Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the antibiotic resistance/susceptibility of the sea snails.Materials and method. The anti-microbial susceptibilities of Gram-negative bacteriawas assessed by the Kirby-Bauer disk diffusion method, the presence of the mcr genes (mcr-1 to-5), the major carbapenemase and 13-lactamase resistant genes in Gram-negative bacteria, using mPCR method and 16S rRNA sequence analysis of A. hydrophila isolates.Results. Bacterial growth accounted for 100% and 94.2% in the samples of intestine and meat, respectively, in the snails. The main organisms identified by MALDI-TOF MS were A. salmonicida subsp. salmonicida at 33.7%, followed by Raoultella ornithinolytica at 9.6% (10/104) and Staphylococcus warneri at 7.7% in meat and intestine samples. Aeromonas hydrophila/ punctata (caviae), Aeromonas sobria, Klebsiella aerogenes, Klebsiella oxytoca, Raoultella planticola, Shewanella putrefaciens and Vibrio vulnificus are intrinsic or chromosomally-mediated resistant against ampicillin. No mcr genes (mcr-1 to-5), the major carbapenemase and 13-lactamase resistant genes were found. Aeromonas salmonicida subsp. salmonicida showed very low levofloxacin and meropenem resistance levels at 2.9%. When the sequence was searched in the Blast database, the genome of A. hydrophila/punctata (caviae) isolate showed high similarity with the A. hydrophila sequences. Conclusions. The findings obtained not only provide data about the proportion of bacteria in the gut and meat of the sea snails and their antibiotic resistance/susceptibility, but also show the absence of carbapenemase, colistin, and 13-lactamase resistant genes among bacterial isolates from sea snail gut microbes.en_US
dc.description.sponsorshipKEGA grant [006UVLF-4/2020]en_US
dc.description.sponsorshipThe study was supported by a KEGA grant No. 006UVLF-4/2020.en_US
dc.identifier.doi10.26444/aaem/163582
dc.identifier.endpage243en_US
dc.identifier.issn1232-1966
dc.identifier.issn1898-2263
dc.identifier.issue2en_US
dc.identifier.pmid37387372en_US
dc.identifier.scopus2-s2.0-85163837488en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage235en_US
dc.identifier.urihttps://doi.org/10.26444/aaem/163582
dc.identifier.urihttps://hdl.handle.net/11616/101439
dc.identifier.volume30en_US
dc.identifier.wosWOS:001030521000005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherInst Rural Health Lublin, Polanden_US
dc.relation.ispartofAnnals of Agricultural and Environmental Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbapenemen_US
dc.subjectcolistinen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectresistance genesen_US
dc.subjectsea snailen_US
dc.titleInvestigation for the presence of bacteria and antimicrobial resistance genes in sea snails (Rapana venosa)en_US
dc.typeArticleen_US

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