Rapid detection of bloodstream pathogens in liver transplantation patients with film array multiplex polymerase chain reaction assays comparison with conventional methods
Yükleniyor...
Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Transplantation Proceedings
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background. Bloodstream infection (BSI) is an important concern in transplant patients.
Early intervention with appropriate antimicrobial therapy is critical to better clinical outcome;
however, there is significant delay when conventional identification methods are used.
Methods. We aimed to determine the diagnostic performance of the FilmArray BloodCulture
Identification Panel, a recently approved multiplex polymerase chain reaction assay detecting
24 BSI pathogens and 3 resistance genes, in comparison with the performances of conventional
identification methods in liver transplant (LT) patients. A total of 52 defined sepsis episodes
(signal-positive by blood culture systems) from 45 LT patients were prospectively studied.
Results. The FilmArray successfully identified 37 of 39 (94.8%) bacterial and 3 of 3
(100%) yeast pathogens in a total of 42 samples with microbial growth, failing to detect
only 2 of 39 (5.1%) bacterial pathogens that were not covered by the test panel. The
FilmArray could also detect additional pathogens in 3 samples that had been reported as
having monomicrobial growth, and it could detect Acinetobacter baumannii in 2 samples
suspected of skin flora contamination. The remaining 8 blood cultures showing a positive
signal but yielding no growth were also negative by this assay. Results of MecA, KPC, and
VanA/B gene detection were in high accordance. The FilmArray produced results with
significantly shorter turnaround times (1.33 versus 36.2, 23.6, and 19.5 h; P < .05) than
standard identification methods, Vitek II, and Vitek MS, respectively.
Conclusions. This study showed that the FilmArray appeared as a reliable alternative
diagnostic method with the potential to mitigate problems with protracted diagnosis of the
BSI pathogens in LT patients.
Açıklama
Anahtar Kelimeler
Kaynak
Transplantation Proceedings
WoS Q Değeri
Scopus Q Değeri
Cilt
47
Sayı
6
Künye
Otlu, B. Bayındır, Y. Özdemir, F. İnce, V. Çuğlan, S. Hopoğlu, M. Yakupoğulları, Y. Kızılkaya, C. Kuzucu, Ç. Işık, B. Yılmaz, S. (2015). Rapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With FilmArray Multiplex Polymerase Chain Reaction Assays Comparison With Conventional Methods. Transplantation Proceedings, 47(6), 1926–1932.
