Rapid detection of bloodstream pathogens in liver transplantation patients with film array multiplex polymerase chain reaction assays comparison with conventional methods

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dc.authorid101949en_US
dc.contributor.authorOtlu, Barış
dc.contributor.authorBayındır, Yaşar
dc.contributor.authorÖzdemir, Fatih
dc.contributor.authorİnce, Volkan
dc.contributor.authorÇuğlan, Serpil
dc.contributor.authorHopoğlu, Murat
dc.contributor.authorYakupoğulları, Yusuf
dc.contributor.authorKızılkaya, Canan
dc.contributor.authorKuzucu, Çiğdem
dc.contributor.authorIşık, Burak
dc.contributor.authorYılmaz, Sezai
dc.date.accessioned2017-07-10T07:49:25Z
dc.date.available2017-07-10T07:49:25Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground. Bloodstream infection (BSI) is an important concern in transplant patients. Early intervention with appropriate antimicrobial therapy is critical to better clinical outcome; however, there is significant delay when conventional identification methods are used. Methods. We aimed to determine the diagnostic performance of the FilmArray BloodCulture Identification Panel, a recently approved multiplex polymerase chain reaction assay detecting 24 BSI pathogens and 3 resistance genes, in comparison with the performances of conventional identification methods in liver transplant (LT) patients. A total of 52 defined sepsis episodes (signal-positive by blood culture systems) from 45 LT patients were prospectively studied. Results. The FilmArray successfully identified 37 of 39 (94.8%) bacterial and 3 of 3 (100%) yeast pathogens in a total of 42 samples with microbial growth, failing to detect only 2 of 39 (5.1%) bacterial pathogens that were not covered by the test panel. The FilmArray could also detect additional pathogens in 3 samples that had been reported as having monomicrobial growth, and it could detect Acinetobacter baumannii in 2 samples suspected of skin flora contamination. The remaining 8 blood cultures showing a positive signal but yielding no growth were also negative by this assay. Results of MecA, KPC, and VanA/B gene detection were in high accordance. The FilmArray produced results with significantly shorter turnaround times (1.33 versus 36.2, 23.6, and 19.5 h; P < .05) than standard identification methods, Vitek II, and Vitek MS, respectively. Conclusions. This study showed that the FilmArray appeared as a reliable alternative diagnostic method with the potential to mitigate problems with protracted diagnosis of the BSI pathogens in LT patients.en_US
dc.identifier.citationOtlu, B. Bayındır, Y. Özdemir, F. İnce, V. Çuğlan, S. Hopoğlu, M. Yakupoğulları, Y. Kızılkaya, C. Kuzucu, Ç. Işık, B. Yılmaz, S. (2015). Rapid Detection of Bloodstream Pathogens in Liver Transplantation Patients With FilmArray Multiplex Polymerase Chain Reaction Assays Comparison With Conventional Methods. Transplantation Proceedings, 47(6), 1926–1932.en_US
dc.identifier.doi10.1016/j.transproceed.2015.02.025en_US
dc.identifier.endpage1932en_US
dc.identifier.issn00411345
dc.identifier.issue6en_US
dc.identifier.startpage1926en_US
dc.identifier.urihttp://linkinghub.elsevier.com/retrieve/pii/S0041134515005011
dc.identifier.urihttps://hdl.handle.net/11616/7353
dc.identifier.volume47en_US
dc.language.isoenen_US
dc.publisherTransplantation Proceedingsen_US
dc.relation.ispartofTransplantation Proceedingsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleRapid detection of bloodstream pathogens in liver transplantation patients with film array multiplex polymerase chain reaction assays comparison with conventional methodsen_US
dc.typeArticleen_US

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