Comparative toxicity of methidathion and glyphosate on early life stages of three amphibian species: Pelophylax ridibundus, Pseudepidalea viridis, and Xenopus laevis

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dc.authoridGüngördü, Abbas/0000-0001-7770-0660
dc.authorwosidGüngördü, Abbas/A-3009-2015
dc.contributor.authorGungordu, Abbas
dc.date.accessioned2024-08-04T20:37:42Z
dc.date.available2024-08-04T20:37:42Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe assessments of pesticide toxicity on nontarget organisms have largely been focused on the determination of median lethal concentration (LC50) values using single/laboratory species. Although useful, these studies cannot describe the biochemical mechanisms of toxicity and also cannot explain the effects of pesticides on natural species. In this study, the toxic effects of glyphosate and methidathion were evaluated comparatively on early developmental stages of 3 anurans-2 natural (Pelophylax ridibundus, Pseudepidalea viridis) and 1 laboratory species (Xenopus laevis). The 96-h LC50 values for methidathion and glyphosate were determined as 25.7-19.6 mg active ingredient (AI)/L for P. viridis, 27.4-22.7 mg AI/L for P. ridibundus, and 15.3-5.05 mg AI/L for X. laevis tadpoles. Furthermore, as early signs of intoxication, glutathione S-transferase (GST), acetylcholinesterase (AChE), carboxylesterase (CaE), glutathione reductase, lactate dehydrogenase, and aspartate aminotrasferase were assayed in 4-day-old tadpoles after 96-h pesticide exposure. The GST induction after 3.2 mg AI/L methidathion exposure was determined to be 173%, 83%, and 38% of control, and the AChE inhibition for the same dose was determined to be 86%, 96%, and 30% of control for P. ridibundus, P. viridis, and X. laevis, respectively. Unlike the application of methidathion, all enzyme activities showed statistically significant increases on glyphosate exposure Compared to controls. However, these increases in enzyme activities were not shown to be parallel with the increase of concentration. The levels of increases of GST and AChE were determined to be 111% and 31% for P. ridibundus, 13% and 51% for P. viridis, and 15% and 36% for X. laevis after 3.2 mg AI/L glyphosate exposure, respectively. The findings of the study suggest that the most sensitive species to pesticide exposure is X laevis. The selected biomarker enzymes AChE, CaE, and GST are useful in understanding the toxic mechanisms of these pesticides in anuran tadpoles as early warning indicators. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.aquatox.2013.06.012
dc.identifier.endpage228en_US
dc.identifier.issn0166-445X
dc.identifier.issn1879-1514
dc.identifier.pmid23831689en_US
dc.identifier.scopus2-s2.0-84880006918en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage220en_US
dc.identifier.urihttps://doi.org/10.1016/j.aquatox.2013.06.012
dc.identifier.urihttps://hdl.handle.net/11616/96127
dc.identifier.volume140en_US
dc.identifier.wosWOS:000324900800023en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAquatic Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPelophylax ridibundusen_US
dc.subjectPseudepidalea viridisen_US
dc.subjectXenopus laevisen_US
dc.subjectMethidathionen_US
dc.subjectGlyphosateen_US
dc.subjectBiomarkeren_US
dc.titleComparative toxicity of methidathion and glyphosate on early life stages of three amphibian species: Pelophylax ridibundus, Pseudepidalea viridis, and Xenopus laevisen_US
dc.typeArticleen_US

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