Overexpression of yeast YLR177w gene provides tolerance to antifungal drug tioconazole

Basit Öğe Kaydı
dc.contributor.authorKoç, Salman
dc.contributor.authorDündar, Muhammed
dc.contributor.authorKoç, Ahmet
dc.contributor.authorIsik, Ozlem Aybuke
dc.date.accessioned2024-08-04T19:42:42Z
dc.date.available2024-08-04T19:42:42Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAim: To find genes that provide resistance to antifungal drug tioconazole by a genomic DNA screening approach. Materials and Methods: Wild-type yeast (BY4741) cells were transformed with a high copy genomic DNA expression library and transformants that can grow on toxic levels of Tioconazole were determined. Library plasmids were isolated from the transformants and sequenced for the identification of potential tioconazole resistance gene(s). Results: Yeast YLR177w gene was isolated as a potential tioconazole resistance gene. Its overexpression led cells to tolerate normally toxic levels of tioconazole. Deletion of the YLR177w gene from the yeast genome made cells more vulnerable to the drug. Conclusion: Yeast YLR177w gene encodes a putative protein of unknown function. Our analyses suggest a potential function for the YLR177w gene for tioconazole resistance.en_US
dc.identifier.doi10.5455/annalsmedres.2022.06.177
dc.identifier.endpage1277en_US
dc.identifier.issn2636-7688
dc.identifier.issue11en_US
dc.identifier.startpage1273en_US
dc.identifier.trdizinid1170773en_US
dc.identifier.urihttps://doi.org/10.5455/annalsmedres.2022.06.177
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1170773
dc.identifier.urihttps://hdl.handle.net/11616/88587
dc.identifier.volume29en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofAnnals of Medical Researchen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleOverexpression of yeast YLR177w gene provides tolerance to antifungal drug tioconazoleen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu