Adaptive and terminal endoplasmic reticulum stress genes methylation levels in Parkinson patients' peripheral blood

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dc.contributor.authorGemici, Yagmur Inalkac
dc.contributor.authorDundar, Muhammed
dc.contributor.authorGozukara, Harika Gozde
dc.contributor.authorKoc, Ahmet
dc.date.accessioned2026-04-04T13:32:57Z
dc.date.available2026-04-04T13:32:57Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractBackground and purpose-Misfolded protein stress has come to the fore among the molecular mechanisms that can cause degeneration. Whereas one of the most important protein of adaptive Endoplasmic Reticulum stress (ERS) is XBP1, CHOP and ASK proteins are associated with apoptosis and terminal ERS. To the best of our knowledge, methylation levels of adaptive and terminal ERS genes in Parkinson's Disease (PD) patients' blood are unknown. We aimed to evaluate if there is a difference in the DNA methylation levels of the ERS related protein-coding genes in peripheral blood of PD patients compared with healthy controls. The clinical significance of these gene methylation levels was evaluated as the second aim. Methods-DNA was isolated from the blood of PD patients (n=23) and controls (n=19). We used a methylation-specific qPCR approach to assess the methylation status of the ERS genes. The correlation between clinical findings and the methylation levels in PD patients were evaluated with appropriate statistical methods. Results-Terminal ERS related genes were statistically significantly hypomethylated in PD (ASK1 p=0.020, and CHOP p<0.001) whereas adaptive ERS gene XBP1's methylation level was not different between groups. Except for XBP1 and MMSE positive, and CHOP and depression negative correlation no correlation was found between clinical markers and methylation levels of the selected genes. (p=0.040, p=0.024), Conclusion-PD patients' peripheral blood methylation levels of adaptive and terminal ERS related genes are significantly different from healthy controls'. While XBP1 is known to be neuroprotective, CHOP and ASK are important proteins in apoptosis, and their methylation differences in peripheral blood provide a clue that they could be used as biomarkers in the future. Therefore, further biomarker and treatment studies should be conducted on these proteins and their pathways.
dc.description.sponsorshipInonu University Scientific Research Projects Unit [TDK-2020-2263]
dc.description.sponsorshipFUNDING INFORMATION-This work was supported by Inonu University Scientific Research Projects Unit with the grant number TDK-2020-2263.
dc.identifier.doi10.18071/isz.78.0417
dc.identifier.issn0019-1442
dc.identifier.issn2498-6208
dc.identifier.issue11-12
dc.identifier.pmid41324539
dc.identifier.scopus2-s2.0-105028827672
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.18071/isz.78.0417
dc.identifier.urihttps://hdl.handle.net/11616/108823
dc.identifier.volume78
dc.identifier.wosWOS:001707715300006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherLiteratura Medica
dc.relation.ispartofIdeggyogyaszati Szemle-Clinical Neuroscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectParkinson's disease
dc.subjectadaptive and terminal ER stress
dc.subjectDNA methylation
dc.subjectXBP1
dc.subjectCHOP
dc.subjectASK
dc.titleAdaptive and terminal endoplasmic reticulum stress genes methylation levels in Parkinson patients' peripheral blood
dc.typeArticle

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