Neuroprotective Effects of Bromelain on Acute Ischemic Stroke in Rats
Küçük Resim Yok
Tarih
2026
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springernature
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Ischemic stroke (IS) is a severe neurological disorder that develops as a result of the interruption of cerebral blood flow and leads to high rates of mortality and morbidity. This study examined the neuroprotective potential of bromelain (Brm) in a rat model of IS induced by middle cerebral artery occlusion (MCAO). Three-month-old male Sprague Dawley rats were divided into four groups: Sham, IS, Brm20 + IS, and Brm40 + IS (n = 10 per group). Brm (20 or 40 mg/kg) or vehicle was administered orally for seven days prior to surgery, followed by 60 min of transient MCAO and 24 h of reperfusion. During reperfusion, neurological deficit scores and rotarod performance were assessed, and infarct volume was determined using 2,3,5-triphenyltetrazolium chloride staining. In the IS group, neurological deficits and infarct volume were significantly increased. The oxidative stress marker malondialdehyde (MDA) and pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6) were elevated, whereas the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the anti-apoptotic protein Bcl-2, were reduced. In addition, apoptotic markers (Bax, caspase-3) and angiogenic markers, including vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9), were increased, while the activity of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) signaling was suppressed. Brm treatment attenuated these pathological alterations by reducing angiogenesis, inflammation, and apoptosis, enhancing antioxidant defense, and restoring PI3K/Akt signaling activity. In conclusion, the present study demonstrated that Brm exerts multifaceted neuroprotective effects in experimental IS by targeting key mechanisms including oxidative stress, inflammation, apoptosis, angiogenesis, and PI3K/Akt signaling. These findings suggest that Brm may represent a potential pharmacological candidate for the treatment of IS, pending validation in future advanced preclinical and clinical studies.
Açıklama
Anahtar Kelimeler
Bromelain, Inflammation, Ischemic Stroke, Neuroprotection, Oxidative stress
Kaynak
Bratislava Medical Journal
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
127
Sayı
2
