Deneysel hipertansiyon modelinde egzersizin nükleer faktör-?b ligandı, osteoprotegerin ve irisin düzeylerine etkisinin araştırılması
Küçük Resim Yok
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: RANK/RANKL/OPG yolağının kardiyovasküler hastalıkların fizyopatolojisinde rol oynadığı bildirilmiştir. Çalışmamızda; RANK, RANKL, OPG ve irisinin hipertansiyon fizyopatolojisindeki rolünü, egzersizin RANK, RANKL, OPG, irisin düzeylerine etkisinin araştırılması amaçlandı. Metaryel ve Metot: Çalışmada Sprague Dawley erkek sıçanlar eşit olarak 4 gruba ayrıldı (n=32). Kontrol, kontrol+egzersiz, hipertansiyon ve hipertansiyon+egzersiz grupları oluşturuldu. Hipertansiyon ve hipertansiyon+ egzersiz gruplarındaki sıçanlara L-NAME (400 mg/L) içme sularına katılarak 6 hafta verildi. Kontrol+egzersiz, hipertansiyon+ egzersiz grubundaki sıçanlara, son 4 hafta boyunca 45 dk süreyle 20 m/dk hızda koşu bandında kronik koşu egzersizi yaptırıldı. Sıçanların 7. hafta ilk gün 24 saatlik idrarları toplandıktan sonra; kan, kas, kalp ve her iki böbreği alınarak ötenazi uygulandı. Bulgular: Kontrol grubuna göre; kontrol+egzersiz grubunda GSH düzeyleri kaslarda azaldı ve kalpte arttı (p<0.05), hipertansiyon grubunda ortalama arteriyel kan basıncı, kalp ve böbrek GSH düzeyleri ile serum irisin düzeyi yükseldi (p<0.05). Hipertansiyon grubuna göre; hipertansiyon+egzersiz grubunda MDA kas düzeyinde artış görüldü (p<0.05). Sonuç: Egzersizin kan basıncını düşürücü etkisinin mekanizması ile RANK/RANK/OPG yolağının hipertansiyon üzerindeki olası mekanizmasının anlaşılabilmesi için L-NAME dozu, verilme süresi ile egzersizin türü, şiddeti, uygulama süresinde yapılacak düzenlemeler sonucu yapılacak yeni çalışmalara ihtiyaç olduğu düşüncesindeyiz. Anahtar kelimeler: Nükleer Faktör-?B Ligandı, Osteoprotegerin, İrisin, Hipertansiyon, Egzersiz
Aim: It has been reported that the RANK/RANKL/OPG pathway plays a role in the pathophysiology of cardiovascular diseases. In our study, we aimed to investigate the role of RANK, RANKL, OPG, and irisin in the pathophysiology of hypertension, and to explore the effect of exercise on the levels of RANK, RANKL, OPG, and irisin. Materials and Methods: Male Sprague Dawley rats were divided equally into 4 groups (n=32): control, control+exercise, hypertension, and hypertension+exercise. Rats in the hypertension and hypertension+exercise groups were given L-NAME (400 mg/L) in their drinking water for 6 weeks. Rats in the control+exercise and hypertension+exercise groups underwent chronic running exercise on a treadmill at a speed of 20 m/min for 45 minutes during the last 4 weeks. On the first day of the 7th week, 24-hour urine samples were collected, and euthanasia was performed to collect blood, muscle, heart, and both kidneys. Results: Compared to the control group, GSH levels decreased in muscles and increased in the heart in the control+exercise group (p<0.05). In the hypertension group, mean arterial blood pressure, heart, and kidney GSH levels, and serum irisin levels increased (p<0.05). Compared to the hypertension group, the hypertension+exercise group showed an increase in MDA levels in muscles (p<0.05). Conclusion: We believe that further studies are needed to understand the mechanism by which exercise lowers blood pressure and the potential mechanism of the RANK/RANK/OPG pathway in hypertension. Adjustments in the dose and duration of L-NAME, as well as the type, intensity, and duration of exercise, should be considered in future studies. Keywords: Nuclear Factor-?B Ligand, Osteoprotegerin, Irisin, Hypertension, Exercise
Aim: It has been reported that the RANK/RANKL/OPG pathway plays a role in the pathophysiology of cardiovascular diseases. In our study, we aimed to investigate the role of RANK, RANKL, OPG, and irisin in the pathophysiology of hypertension, and to explore the effect of exercise on the levels of RANK, RANKL, OPG, and irisin. Materials and Methods: Male Sprague Dawley rats were divided equally into 4 groups (n=32): control, control+exercise, hypertension, and hypertension+exercise. Rats in the hypertension and hypertension+exercise groups were given L-NAME (400 mg/L) in their drinking water for 6 weeks. Rats in the control+exercise and hypertension+exercise groups underwent chronic running exercise on a treadmill at a speed of 20 m/min for 45 minutes during the last 4 weeks. On the first day of the 7th week, 24-hour urine samples were collected, and euthanasia was performed to collect blood, muscle, heart, and both kidneys. Results: Compared to the control group, GSH levels decreased in muscles and increased in the heart in the control+exercise group (p<0.05). In the hypertension group, mean arterial blood pressure, heart, and kidney GSH levels, and serum irisin levels increased (p<0.05). Compared to the hypertension group, the hypertension+exercise group showed an increase in MDA levels in muscles (p<0.05). Conclusion: We believe that further studies are needed to understand the mechanism by which exercise lowers blood pressure and the potential mechanism of the RANK/RANK/OPG pathway in hypertension. Adjustments in the dose and duration of L-NAME, as well as the type, intensity, and duration of exercise, should be considered in future studies. Keywords: Nuclear Factor-?B Ligand, Osteoprotegerin, Irisin, Hypertension, Exercise
Açıklama
Sağlık Bilimleri Enstitüsü, Fizyoloji Ana Bilim Dalı
Anahtar Kelimeler
Fizyoloji, Physiology
