Modulation of the dual-faced effects of miR-141 with chitosan/miR-141 nanoplexes in breast cancer cells

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dc.authoridKaban, Kübra/0000-0001-7311-7187
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authoridAKBUGA, JULIDE/0000-0002-1693-9579
dc.authorwosidKaban, Kübra/JFB-1837-2023
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.contributor.authorKaban, Kubra
dc.contributor.authorSalva, Emine
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:46:44Z
dc.date.available2024-08-04T20:46:44Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground miR-141, known as a tumor suppressive microRNA, is downregulated in breast cancer. However, recent contrasting studies report that it also acts as oncogene when it is upregulated. The present study aimed to investigate whether miR-141 is a tumor suppressor or oncogenic when it reaches normal levels in chitosan/miR-141 nanoplexes. Methods Chitosan nanoplexes were prepared using simple complexation method. Nanoplexes were characterized by a gel retardation assay and zeta potential and particle size measurements. To determine the expression level of miR-141, a quantitative real-time polymerase chain reaction was performed. The effects of miR-141 mimics were investigated with respect to angiogenesis by vascular endothelial growth factor (VEGF), epithelial-mesenchymal transition (EMT) by E-cadherin, metastasis by Igfbp-4 and Tinagl1 enzyme-linked immunosorbent assays, invasion by an invasion chamber, and apoptosis by Annexin V. Results The miR-141 expression levels of MDA-MB-231 and MDA-MB-435 cells by administration of chitosan/mimic miR-141 nanoplexes reached endogenous miR-141 levels of a non-tumorigenic epithelial breast cell line, MCF-10A. According to our results, metastasis, VEGF, EMT and invasion in breast cancer cells were diminished, whereas apoptosis increased by 1.5- and 2.4-fold in breast cancer cell lines as a result of the miR-141 mimics. Conclusions In conclusion, we have demonstrated that administration of miR-141 mimics at the determined doses to breast cancer cells revealed a tumor suppressor effect, and not the oncogenic face. The delivery of miR-141 by chitosan nanoplexes presents a promising approach for the suppression of breast cancer.en_US
dc.identifier.doi10.1002/jgm.3116
dc.identifier.issn1099-498X
dc.identifier.issn1521-2254
dc.identifier.issue9en_US
dc.identifier.pmid31389101en_US
dc.identifier.scopus2-s2.0-85070956949en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/jgm.3116
dc.identifier.urihttps://hdl.handle.net/11616/98919
dc.identifier.volume21en_US
dc.identifier.wosWOS:000483371400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Gene Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbreast cancer cellsen_US
dc.subjectchitosan nanoplexesen_US
dc.subjectdouble-faced miRNAen_US
dc.subjectmiR-141en_US
dc.titleModulation of the dual-faced effects of miR-141 with chitosan/miR-141 nanoplexes in breast cancer cellsen_US
dc.typeArticleen_US

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