Highly stable laccase from repeated-batch culture of Funalia trogii ATCC 200800

Basit Öğe Kaydı
dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authoridOzmen, Nesrin/0000-0003-1080-3360;
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.authorwosidOzmen, Nesrin/ABH-8404-2020
dc.authorwosidBirhanli, Emre/AAA-4009-2021
dc.contributor.authorYesilada, O.
dc.contributor.authorBirhanli, E.
dc.contributor.authorOzmen, N.
dc.contributor.authorErcan, S.
dc.date.accessioned2024-08-04T20:38:02Z
dc.date.available2024-08-04T20:38:02Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe effect of temperature, pH, different inhibitors and additives on activity and stability of crude laccase obtained from repeated-batch culture of white rot fungus Funalia trogii ATCC 200800 was studied. The crude enzyme showed high activity at 55-90A degrees C, which was maximal at 80-95A degrees C. It was highly stable within the temperature intervals 20-50A degrees C. The half life of the enzyme was about 2 h and 5 min at 60A degrees C and 70A degrees C, respectively. pH optimum of fungal laccase activity was revealed at pH 2.5. The enzyme from F. trogii ATCC 200800 was very stable between pH values of 3.0-9.0. NaN3 and KCN were detected as the most effective potent enzyme inhibitors among different compounds tested. The fungal enzyme was highly resistant to the various metal ions, inorganic salts, and organic solvents except propanol, at least for 5 min. Because of its high stability and efficient decolorization activity, the use of the crude F. trogii ATCC 200800 laccase instead of pure enzyme form may be a considerably cheaper solution for biotechnological applications.en_US
dc.description.sponsorshipInonu University Research Fund Unit [2010/127]en_US
dc.description.sponsorshipWe would like to thank Inonu University Research Fund Unit for their financial support throughout this study (2010/127).en_US
dc.identifier.doi10.1134/S0003683814010128
dc.identifier.endpage61en_US
dc.identifier.issn0003-6838
dc.identifier.issn1573-8183
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84893614912en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage55en_US
dc.identifier.urihttps://doi.org/10.1134/S0003683814010128
dc.identifier.urihttps://hdl.handle.net/11616/96338
dc.identifier.volume50en_US
dc.identifier.wosWOS:000329100800009en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofApplied Biochemistry and Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectThermostable Laccasesen_US
dc.subjectFungal Laccasesen_US
dc.subjectSynthetic Dyesen_US
dc.subjectTextile Dyesen_US
dc.subjectDecolorizationen_US
dc.subjectPurificationen_US
dc.subjectDecolourizationen_US
dc.subjectRecalcitranten_US
dc.subjectOptimizationen_US
dc.subjectAtcc-200800en_US
dc.titleHighly stable laccase from repeated-batch culture of Funalia trogii ATCC 200800en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu