Cytotoxicity Evaluation of Bioresorbable Fixation Screws on Human Gingival Fibroblasts and Mouse Osteoblasts by Real-Time Cell Analysis

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dc.authoridYolcu, Ümit/0000-0001-7312-2867
dc.authoridHakkı, Sema S/0000-0001-8665-6235
dc.authorwosidYolcu, Ümit/ABH-6916-2020
dc.authorwosidalan, hilal/ABH-9301-2020
dc.authorwosidHakkı, Sema S/Y-2878-2018
dc.contributor.authorYolcu, Umit
dc.contributor.authorAlan, Hilal
dc.contributor.authorMalkoc, Siddik
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorHakki, Sema Sezgin
dc.date.accessioned2024-08-04T20:40:23Z
dc.date.available2024-08-04T20:40:23Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurpose: To evaluate the effects of bioresorbable fixation screws (BFSs) on human gingival fibroblast (HGF) and mouse osteoblast (MC3T3-E1) cell viability. Materials and Methods: The KLS Martin SonicPins Rx, Synthes RapidSorb Cortex Screws, and Inion CPS Bioabsorbable Fixation System each were incubated in Dulbecco's Modified Eagle Medium for 72 hours according to ISO 10993-5 standards. A real-time cell analyzer was used to evaluate cell survival. After seeding 200-mu L cell suspensions in the wells of an E-plate View 96, HGF and MC3T3-E1 cells were treated with the bioactive components released by the bioresorbable materials and monitored every 15 minutes for 96 hours. Statistical significance was determined using 1-way analysis of variance and Tukey-Kramer tests. Results: There were significant differences in the HGF responses to the untreated control conditions and the Synthes (P < .01), Inion (P < .05), and KLS Martin (P < .05) treatments over 48 hours. The Synthes (P < .01) and Inion (P < .01) treatments produced lower HGF cell index values than the untreated control at 72 hours, whereas the KLS Martin treatment did not. When left to elute for 96 hours, there were no significant differences in values among the control and study groups for HGFs (P > .05). All tested BFSs decreased cell survival rates of M3T3C1 cells for 48 hours (P < .01), 72 hours (P < .001), and 96 hours (P < .001). Conclusion: Differences in the sensitivities of the 2 tested cell lines to the different BFSs might be the result of the different materials used to manufacture the screws. These results provide fundamental knowledge and new insights for the future design and development of new biocompatible BFSs for oral and maxillofacial surgery. (C) 2015 American Association of Oral and Maxillofacial Surgeonsen_US
dc.identifier.doi10.1016/j.joms.2015.03.067
dc.identifier.issn0278-2391
dc.identifier.issn1531-5053
dc.identifier.issue8en_US
dc.identifier.pmid25930954en_US
dc.identifier.scopus2-s2.0-84938651044en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.joms.2015.03.067
dc.identifier.urihttps://hdl.handle.net/11616/96892
dc.identifier.volume73en_US
dc.identifier.wosWOS:000357878600019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofJournal of Oral and Maxillofacial Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn-Vitroen_US
dc.subjectBioabsorbable Fixationen_US
dc.subjectShort-Termen_US
dc.subjectImplantsen_US
dc.subjectBiocompatibilityen_US
dc.subjectProliferationen_US
dc.subjectFracturesen_US
dc.subjectCopolymeren_US
dc.subjectSurgeryen_US
dc.subjectSystemen_US
dc.titleCytotoxicity Evaluation of Bioresorbable Fixation Screws on Human Gingival Fibroblasts and Mouse Osteoblasts by Real-Time Cell Analysisen_US
dc.typeArticleen_US

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