Candida albicans outbreak associated with total parenteral nutrition in the neonatal unit
Yükleniyor...
Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Indian Journal of Medical Microbiology
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: The most frequently isolated fungi in patients using TPN belongs to the Candida genus. Various infections
including venous catheter infections, fungemia, endocarditis and ophthalmitis may be encountered. Objective: Upon
growth of Candida in the blood cultures from the pediatric (neonatal) unit of our hospital, a surveillance was performed
in this unit and involving the health care workers. Clonal relationships of the isolates were investigated with molecular
tests. Methods: Blood samples obtained from the patients in pediatric neonatal unit were studied with automatized blood
culture [BacT/Alert (Bio Mérioux, France)]. Yeast isolates from environmental surveillance cultures (TPN solutions,
hands of healthcare personnel, étagère, etc) and patients were identified as C. albicans with conventional methods and
ID 32 C and ATBTM Fungus 3 (Biomerieux, France) kits. Clonal similarity was determined by using AP-PCR as initial
method and we have also typified all strains by the method of REP-PCR (diversilab system,bioMérieux). Finally; Pulsed
Field Gel Electrophoresis (PFGE) was used for confirmation. Results: C. albicans was isolated in blood cultures of
seven patients. Similar antifungal susceptibility patterns were observed in all isolates. AP-PCR and REP-PCR showed
that the C. albicans isolates grown in the TPN solution and from the patients’ blood cultures were clonally same strains.
PFGE analysis further confirmed this clonality. Conclusion: According to results of the molecular methods, we thought
that a C. albicans outbreak had occurred in the neonatal pediatric unit, due to contamination of TPN solution.
Açıklama
Anahtar Kelimeler
Candida albicans, Fungal infection, Neonatal unit, Parenteral nutrition
Kaynak
Indian Journal of Medical Microbiology
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
2
Künye
Güdücüoğlu, H. Gültepe, B. S. Otlu, B. Bektaş, H. Yıldırım, Ö. Tuncer, O. Berktaş, M. (2016). Candida albicans outbreak associated with total parenteral nutrition in the neonatal unit. Indian Journal of Medical Microbiology, 34(2), 202–207.
