Inhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitor

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dc.authoridKARAKUŞ, Fuat/0000-0002-5260-3650
dc.authoridÜnüvar, Songül/0000-0001-8454-490X
dc.authorwosidKARAKUŞ, Fuat/O-2627-2019
dc.authorwosidÜnüvar, Songül/ABH-5516-2020
dc.contributor.authorKarakus, Fuat
dc.contributor.authorEyol, Ergul
dc.contributor.authorYilmaz, Kadir
dc.contributor.authorUnuvar, Songul
dc.date.accessioned2024-08-04T20:45:43Z
dc.date.available2024-08-04T20:45:43Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion. Objectives: In the present study, we aimed to investigate the effect of acetazolamide, which is a classical carbonic anhydrase inhibitor, on the cell viability, migration, and colony forming capacity of human LS174T colorectal cancer cells. Methods: Three different cell culture techniques (MTT test, wound healing and clonogenic assay) were performed in this in vitro study on colorectal cancer cells. Results: Acetazolamide reduced the cell viability, migration and colony formation ability of cells depending on dose. There was no significant difference between the cells treated with acetazolamide with 1 mu M dose and the control. However, it can be concluded that acetazolamide exerts its effect on human colorectal cancer cells at 10-1000 mu M concentrations. Conclusion: Acetazolamide was observed to significantly inhibit the cell viability, colony forming capacity, and migration ability in the culture medium of LS174T cells. This inhibitor effect of acetazolamide was observed to be dependent on the concentration in medium.en_US
dc.description.sponsorshipInonu University Scientific Research Projects Coordination Unit [2014/29]en_US
dc.description.sponsorshipThe study was supported by Inonu University Scientific Research Projects Coordination Unit with project number 2014/29. The authors would like to thank Prof. Dr. Martin R Berger (The German Cancer Research Center, Heidelberg, Germany).en_US
dc.identifier.doi10.4314/ahs.v18i4.51
dc.identifier.endpage1310en_US
dc.identifier.issn1680-6905
dc.identifier.issn1729-0503
dc.identifier.issue4en_US
dc.identifier.pmid30766596en_US
dc.identifier.scopus2-s2.0-85061562282en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1303en_US
dc.identifier.urihttps://doi.org/10.4314/ahs.v18i4.51
dc.identifier.urihttps://hdl.handle.net/11616/98656
dc.identifier.volume18en_US
dc.identifier.wosWOS:000451941800051en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMakerere Univ, Fac Meden_US
dc.relation.ispartofAfrican Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLS174Ten_US
dc.subjectcarbonic anhydrase inhibitoren_US
dc.subjectcolorectal carcinomaen_US
dc.titleInhibition of cell proliferation, migration and colony formation of LS174T Cells by carbonic anhydrase inhibitoren_US
dc.typeArticleen_US

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