Random/aligned electrospun PCL fibrous matrices with modified surface textures: Characterization and interactions with dermal fibroblasts and keratinocytes

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dc.authorwosidŞimşek, Murat/ABF-3212-2020
dc.contributor.authorPoyraz, Seyma
dc.contributor.authorAltinisik, Zeynep
dc.contributor.authorCakmak, Anil Sera
dc.contributor.authorSimsek, Murat
dc.contributor.authorGumusderelioglu, Menemse
dc.date.accessioned2024-08-04T20:52:14Z
dc.date.available2024-08-04T20:52:14Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMicro- or nano-surface topography of a biomaterial can improve various cellular activities for obtaining functional tissues. Electrospun fibers can gain further functionality when introduced topographic details to their surfaces. In this regard, we produced random and aligned polycaprolactone (PCL) micron/submicron fibers by the electrospinning method. Simultaneously, the surface structure of the fibers was altered by applying phase separation processes including non-solvent-induced phase separation (NIPS) and vapor-induced phase separation (VIPS) mechanisms. As a result, PCL fibers with porous, wrinkled, grooved, and crater-like morphology were obtained. Human dermal fibroblasts (BJ cells) and human keratinocytes (HS2) were cultured onto the fiber surfaces and the data were evaluated in terms of cell-material interactions. Results showed that not only the orientation of fibers but also fiber topography affected both cell-fiber and cell-cell interactions in different manners. It was observed that the wrinkled topography is the most suitable for both dermal fibroblasts and keratinocytes in terms of cell attachment and proliferation. We also concluded that cellular behavior was varied according to the morphology of the cells used. Morphological observations showed that HS2 cells proliferated more intensively on all surfaces compared to BJ cells. All these findings can be evaluated in terms of the design of tissue scaffolds, especially in skin tissue engineering.en_US
dc.description.sponsorshipHacettepe and Inonu University Scientific Researches Project units [FYL-2020-18725, FBA-2018-1332]en_US
dc.description.sponsorshipThis work was supported by Hacettepe and Inonu University Scientific Researches Project units under project numbers FYL-2020-18725 and FBA-2018-1332, respectively.en_US
dc.identifier.doi10.1016/j.colsurfb.2022.112724
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid35961110en_US
dc.identifier.scopus2-s2.0-85135688033en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2022.112724
dc.identifier.urihttps://hdl.handle.net/11616/100830
dc.identifier.volume218en_US
dc.identifier.wosWOS:000888203700001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofColloids and Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNanotopographyen_US
dc.subjectPCLen_US
dc.subjectElectrospinningen_US
dc.subjectPhase separation mechanismsen_US
dc.subjectHuman dermal fibroblastsen_US
dc.subjectHuman keratinocytesen_US
dc.titleRandom/aligned electrospun PCL fibrous matrices with modified surface textures: Characterization and interactions with dermal fibroblasts and keratinocytesen_US
dc.typeArticleen_US

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