Resveratrolün ağır egzersiz yaptırılan sıçanlarda kalp ve çizgili kaslardaki endoplazmik retikulum stresi üzerine etkisi
Küçük Resim Yok
Tarih
2024
Yazarlar
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Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Ağır egzersiz endoplazmik retikulum stresine neden olabilir. Bu tez çalışmasının amacı resveratrolün ağır egzersiz sonucu çizgili ve miyokart kasında oluşan hasar mekanizmalarından birisi olan ER stresi üzerindeki etkinliğini araştırmaktır. Materyal ve metot: Sıçanlar, Kontrol (n=10), Şiddetli Yüzme Egzersizi (n=10) (H-Ex), Şiddetli Yüzme Egzersizi + Resveratrol (n=10) (H-Ex+RES) olacak şekilde üç gruba ayrıldı. H-Ex ve H-Ex+RES gruptaki sıçanlar, yüzme süreleri 2 haftada bir 10 dk artacak şekilde 10 hafta yüzdürüldü. H-Ex ve H-Ex+RES gruptaki sıçanlara ilk hafta vücut ağırlığının %5'i oranında yük bağlanarak ve 2 haftada bir %1 oranında artırılarak yüzdürüldü. H-Ex+RES grubuna çalışmanın son 4 haftasında resveratrol (10 mg/kg/gün) verildi. Western Blot metodu ile sol ventrikül ve gastrocnemius kasında ER stres belirteçlerinden GRP78, XBP1, ATF4 protein düzeyleri ölçüldü. Veriler uygun istatistiksel yöntemlerle değerlendirildi. Bulgular: GRP78 ve ATF4 düzeylerinin H-Ex grubunda hem sol ventrikül kasında hem de gastrocnemius kasında arttı (p<0.05). H-Ex'de, XBP1 seviyesi sol ventrikülde azalırken gastrocnemiusta ise arttı (p<0.05). H-Ex+RES grubunda ise resveratrol her iki dokuda GRP78 ve ATF4 yükselmesini engellediğini ancak sol ventrikülde XBP1'in hem kontrol hem de H-Ex gruplarına göre daha düşük düzeyde olmasına neden oldu (p<0.05). Sonuç: Yoğun egzersizin hem kalp hem de iskelet kasında ER stresini artırdığı görülürken resveratrolün ER stresinin azaltılması yönünde oldukça yararlı olduğu sonucuna varıldı. Anahtar Kelimeler: Aktive Edici Transkripsiyon Faktörü 4, Endoplazmik Retikulum Stresi, Glikoz Regüle Edici Protein 78, Resveratrol, Yüzme egzersizi, X-box Bağlayıcı Protein 1
Aim: Heavy exercise may cause endoplasmic reticulum stress. The aim of this thesis was to investigate the efficacy of resveratrol on ER stress, one of the mechanisms of damage in striated and myocardial muscle caused by heavy exercise. Material and Method: Rats were divided into three groups as Control (n=10), High intensive Exercise (n=10) (H-Ex), High intensive Exercise + Resveratrol (n=10) (H-Ex+RES). Rats in the H-Ex and H-Ex+RES groups were made to swim for 10 weeks, with swimming times increasing by 10 min every 2 weeks. Rats in H-Ex and H-Ex+RES groups were loaded with 5% of their body weight in the first week and their swimming time was increased by 1% every 2 weeks. Resveratrol (10 mg/kg/day) was given to H-Ex+RES group in the last 4 weeks of the study. Protein levels of ER stress markers GRP78, XBP1, ATF4 were measured in left ventricle and gastrocnemius muscle by Western blotting. Data were analyzed by appropriate statistical methods. Results: GRP78 and ATF4 levels increased in both left ventricular and gastrocnemius muscle in H-Ex group (p<0.05). In the H-Ex, XBP1 levels decreased in the left ventricle and increased in gastrocnemius muscle (p<0.05). In the H-Ex+RES group, resveratrol inhibited the elevation of GRP78 and ATF4 in both tissues but caused a lower level of XBP1 in the left ventricle compared to both control and H-Ex groups (p<0.05). Conclusion: While intense exercise increased ER stress in both cardiac and skeletal muscle, resveratrol was found to be highly beneficial in reducing ER stress. Key Words: Activating Transcription Factor 4, Endoplasmic Reticulum Stress, Glucose Regulating Protein 78, Resveratrol, Swimming exercise, X-box Binding Protein 1
Aim: Heavy exercise may cause endoplasmic reticulum stress. The aim of this thesis was to investigate the efficacy of resveratrol on ER stress, one of the mechanisms of damage in striated and myocardial muscle caused by heavy exercise. Material and Method: Rats were divided into three groups as Control (n=10), High intensive Exercise (n=10) (H-Ex), High intensive Exercise + Resveratrol (n=10) (H-Ex+RES). Rats in the H-Ex and H-Ex+RES groups were made to swim for 10 weeks, with swimming times increasing by 10 min every 2 weeks. Rats in H-Ex and H-Ex+RES groups were loaded with 5% of their body weight in the first week and their swimming time was increased by 1% every 2 weeks. Resveratrol (10 mg/kg/day) was given to H-Ex+RES group in the last 4 weeks of the study. Protein levels of ER stress markers GRP78, XBP1, ATF4 were measured in left ventricle and gastrocnemius muscle by Western blotting. Data were analyzed by appropriate statistical methods. Results: GRP78 and ATF4 levels increased in both left ventricular and gastrocnemius muscle in H-Ex group (p<0.05). In the H-Ex, XBP1 levels decreased in the left ventricle and increased in gastrocnemius muscle (p<0.05). In the H-Ex+RES group, resveratrol inhibited the elevation of GRP78 and ATF4 in both tissues but caused a lower level of XBP1 in the left ventricle compared to both control and H-Ex groups (p<0.05). Conclusion: While intense exercise increased ER stress in both cardiac and skeletal muscle, resveratrol was found to be highly beneficial in reducing ER stress. Key Words: Activating Transcription Factor 4, Endoplasmic Reticulum Stress, Glucose Regulating Protein 78, Resveratrol, Swimming exercise, X-box Binding Protein 1
Açıklama
Anahtar Kelimeler
Fizyoloji, Physiology
