Development of phytase targeted PLGA nanoparticles loaded with albendazole sulfoxide and praziquantel and evaluation of efficacy on the hydatid cysts

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dc.contributor.authorGultekin, Yakup
dc.contributor.authorKorkmaz, Cagla
dc.contributor.authorOzturk, Naile
dc.contributor.authorFilazi, Ayhan
dc.contributor.authorDeniz, Ahmet
dc.contributor.authorVural, Imran
dc.date.accessioned2026-04-04T13:35:00Z
dc.date.available2026-04-04T13:35:00Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractCystic echinococcosis is the metacestode stage of Echinococcus granulosus and is a worldwide public health problem. Today, various difficulties in the treatment of cystic echinococcosis limit the treatment of cystic echinococcosis. In this study, the in vitro activity of PLGA nanoparticles loaded with albendazole sulfoxide and praziquantel and targeted with phytase enzyme on Echinococcus granulosus micro-hydatid cysts was evaluated as a new formulation. For this purpose, first conjugation of PLGA polymer and phytase enzyme was performed, then albendazole sulfoxide and praziquantel loaded nanoparticles were prepared using PLGA-phytase polymer and characterization studies were performed. Permeability and cytotoxicity tests were carried out with cell culture studies of the formulations for which characterization studies were performed and it was found that the formulations did not show any cytotoxic effect. In order to evaluate the formulations, micro-hydatid cysts were developed in vitro with Echinococcus granulosus protoscoleces collected from slaughterhouse animals and the formulations were applied. In vitro hydatid cyst viability studies, approximately 50 % of cysts died on day 4 in the group applied with the combination of 10 mu g/mL free albendazole sulfoxide and 50 mu g/mL free praziquantel, while 100% of cysts died in the group applied with the new formulation containing the same amount of drug. In addition, ex vivo studies on the laminar layer of hydatid cysts obtained from sheep liver showed that the new formulation binds to the laminar layer and increases the permeability of albendazole sulfoxide and praziquantel across the laminar layer by approximately 2-fold.
dc.description.sponsorshipHacettepe University Scientific Research Projects Coordination [UnitwithgrantnumberTSA-2021-19166]; [TSA-2021-19166]
dc.description.sponsorshipThisstudywassupportedby the HacettepeUniversityScientificResearchProjectsCoordination [UnitwithgrantnumberTSA-2021-19166]
dc.identifier.doi10.1016/j.jddst.2025.106662
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.orcid0000-0002-2800-6215
dc.identifier.orcid0000-0001-6504-0757
dc.identifier.scopus2-s2.0-85216673689
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2025.106662
dc.identifier.urihttps://hdl.handle.net/11616/109548
dc.identifier.volume105
dc.identifier.wosWOS:001422958900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Drug Delivery Science and Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectAlbendazole sulfoxide
dc.subjectHydatid cyst
dc.subjectPhytase enzyme
dc.subjectPraziquantel
dc.subjectTargeted nanoparticles
dc.titleDevelopment of phytase targeted PLGA nanoparticles loaded with albendazole sulfoxide and praziquantel and evaluation of efficacy on the hydatid cysts
dc.typeArticle

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