Proteomic changes during boron tolerance in barley Hordeum vulgare and role of vacuolar proton translocatingATPase subunit E
Yükleniyor...
Dosyalar
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high
concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley
(Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen
diff erentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were
revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like
protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding
protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein.
Th ese were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase
subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression
of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. Th ese results indicated that the
V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in
the overall boron tolerance of barley.
Açıklama
Anahtar Kelimeler
Boron stress, Hordeum vulgare, Saccharomyces cerevisiae, Proteomics, V-ATPase
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Ahmet, A., Kaya, A., Akıncı, E., Yalçın, T., Koç, A., Ozan, B., Karakaya, H. Ç. (2011). Proteomic Changes During Boron Tolerance İn Barley Hordeum Vulgare And Role Of Vacuolar Proton Translocatingatpase Subunit E . Tj Bot, (135), 379–388.
