Proteomic changes during boron tolerance in barley Hordeum vulgare and role of vacuolar proton translocatingATPase subunit E
| dc.authorid | 130773 | en_US |
| dc.authorid | 130618 | en_US |
| dc.authorid | 110769 | en_US |
| dc.contributor.author | Atik, Ahmet Emin | |
| dc.contributor.author | Bozdağ, Gönensin Ozan | |
| dc.contributor.author | Akıncı, Ersin | |
| dc.contributor.author | Kaya, Alaattin | |
| dc.contributor.author | Koç, Ahmet | |
| dc.contributor.author | Yalçın, Talat | |
| dc.contributor.author | Karakaya, Hüseyin Çağlar | |
| dc.date.accessioned | 2017-06-10T11:53:16Z | |
| dc.date.available | 2017-06-10T11:53:16Z | |
| dc.date.issued | 2011 | |
| dc.department | Enstitüler, | en_US |
| dc.description.abstract | Boron is an essential micronutrient for plants and animals; however, it can be toxic when present at high concentrations. Th e purpose of this study was to understand the mechanisms of boron tolerance in the Turkish barley (Hordeum vulgare) Anadolu cultivar. For this purpose, 2-dimensional electrophoresis (2-DE) was used to screen diff erentially expressed proteins for both control and boron-stressed Anadolu barley genotypes. Seven proteins were revealed by 2-DE: 1) ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCo large chain), 2) TLP5, a thaumatin-like protein, 3) PR5, a basic pathogenesis-related protein, 4) a RNase S-like protein, 5) a PSI type III chlorophyll a/b-binding protein, 6) a light-harvesting complex I LHC I, and 7) the vacuolar proton-translocating ATPase subunit E protein. Th ese were found to be upregulated in response to boron treatment. Even though the protein encoded by the V-ATPase subunit E gene was overexpressed, its transcript level was downregulated by boron treatment. Heterologous expression of the barley V-ATPase subunit E gene in yeast provided boron resistance to yeast cells. Th ese results indicated that the V-ATPase subunit E gene was functional and conferred tolerance to toxic boron levels in yeast and might play a role in the overall boron tolerance of barley. | en_US |
| dc.identifier.citation | Ahmet, A., Kaya, A., Akıncı, E., Yalçın, T., Koç, A., Ozan, B., Karakaya, H. Ç. (2011). Proteomic Changes During Boron Tolerance İn Barley Hordeum Vulgare And Role Of Vacuolar Proton Translocatingatpase Subunit E . Tj Bot, (135), 379–388. | en_US |
| dc.identifier.endpage | 388 | en_US |
| dc.identifier.startpage | 379 | en_US |
| dc.identifier.uri | https://hdl.handle.net/11616/7077 | |
| dc.language.iso | eng | en_US |
| dc.publisher | İnönü Üniversitesi | en_US |
| dc.relation.publicationcategory | Tez | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Boron stress | en_US |
| dc.subject | Hordeum vulgare | en_US |
| dc.subject | Saccharomyces cerevisiae | en_US |
| dc.subject | Proteomics | en_US |
| dc.subject | V-ATPase | en_US |
| dc.title | Proteomic changes during boron tolerance in barley Hordeum vulgare and role of vacuolar proton translocatingATPase subunit E | en_US |
| dc.type | Master Thesis | en_US |
