Degradation of benzene, toluene and xylene by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin gene

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dc.authoridKahraman, Hüseyin/0000-0001-6235-5497
dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidKahraman, Hüseyin/ABG-9650-2020
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorKahraman, H
dc.contributor.authorGeckil, H
dc.date.accessioned2024-08-04T20:15:02Z
dc.date.available2024-08-04T20:15:02Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study concerns the potential use of Pseudomonas aeruginosa expressing the Vitreoscilla hemoglobin gene for the degradation of important harmful aromatic compounds such as benzene, toluene, and xylene (BTX). The use of these compounds by both strains was determined as the production of cell mass (viable cell number) in a minimal medium containing any one of the BTX compounds as the sole carbon and energy source. Furthermore, the BTX degradation capability of both strains was monitored by measuring the production of 3-methylcatechol, a common intermediate. For the cells of the logarithmic phase, which were grown at high aeration/high agitation or low aeration/low agitation, the engineered strain showed a better growth rate than the host strain. With the benzene in the medium, the recombinant strain exhibited a higher (up to 4-fold) cell density than the parental wild-type strain at this phase. In contrast, regarding the cells of the late stationary phase under high aeration/high agitation conditions, the host strain had generally higher viable cell numbers than the recombinant strain. At this phase this difference was, however, less significant under the conditions of low aeration/low agitation. Similarly, in toluene containing medium (at high aeration/high agitation) the recombinant strain showed a higher cell density which was from a 15-fold to almost one order of magnitude greater than its parental strain during the logarithmic phase where the cell density of P aeruginosa remained nearly constant. Contrary to the results with benzene and toluene, both strains exhibited similar growth characteristics when they were grown in the presence of xylene. The positive effect of the oxygen uptake by the recombinant system on the BTX metabolizing activity was also apparent in a high accumulation of 3-methylcatechol in the cultures of the recombinant strain. At certain points of incubation, the hemoglobin expressing strain showed a significantly (p < 0.05) higher 3-methylcatechol accumulation than the host strain. These results demonstrated the possible potential of the Vitreoscilla hemoglobin as an efficient oxygen uptake system for the bioremediation of some compounds of environmental concern.en_US
dc.identifier.doi10.1002/elsc.200520088
dc.identifier.endpage368en_US
dc.identifier.issn1618-0240
dc.identifier.issn1618-2863
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-25444493886en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage363en_US
dc.identifier.urihttps://doi.org/10.1002/elsc.200520088
dc.identifier.urihttps://hdl.handle.net/11616/94112
dc.identifier.volume5en_US
dc.identifier.wosWOS:000232054900007en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofEngineering in Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject2,4-Dinitrotoluene Degradationen_US
dc.subjectEscherichia-Colien_US
dc.subjectBurkholderia Spen_US
dc.subjectPutidaen_US
dc.subjectBiodegradationen_US
dc.subjectEnhancementen_US
dc.subjectBacteriaen_US
dc.subject3-Methylcatecholen_US
dc.subjectExpressionen_US
dc.subjectHexadecaneen_US
dc.titleDegradation of benzene, toluene and xylene by Pseudomonas aeruginosa engineered with the Vitreoscilla hemoglobin geneen_US
dc.typeArticleen_US

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