Enhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin gene

dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidStark, Benjamin C/E-6095-2010
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorGeckil, H
dc.contributor.authorBarak, Z
dc.contributor.authorChipman, DM
dc.contributor.authorErenler, SO
dc.contributor.authorWebster, DA
dc.contributor.authorStark, BC
dc.date.accessioned2024-08-04T20:56:13Z
dc.date.available2024-08-04T20:56:13Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMicrobial production of butanediol and acetoin has received increasing interest because of their diverse potential practical uses. Although both products are fermentative in nature, their optimal production requires a low level of oxygen. In this study, the use of a recombinant oxygen uptake system on production of these metabolites was investigated. Enterobacter aerogenes was transformed with a pUC8-based plasmid carrying the gene (vgb) encoding Vitreoscilla (bacterial) hemoglobin (VHb). The presence of vgb and production of VHb by this strain resulted in an increase in viability from 72 to 96 h in culture, but no overall increase in cell mass. Accumulation of the fermentation products acetoin and butanediol were enhanced (up to 83%) by the presence of vgb/VHb. This vgb/VHb related effect appears to be due to an increase of flux through the acetoin/butanediol pathway, but not at the expense of acid production.en_US
dc.identifier.doi10.1007/s00449-004-0373-1
dc.identifier.endpage330en_US
dc.identifier.issn1615-7591
dc.identifier.issue5en_US
dc.identifier.pmid15309606en_US
dc.identifier.scopus2-s2.0-8744260557en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage325en_US
dc.identifier.urihttps://doi.org/10.1007/s00449-004-0373-1
dc.identifier.urihttps://hdl.handle.net/11616/102135
dc.identifier.volume26en_US
dc.identifier.wosWOS:000224618700005en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBioprocess and Biosystems Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectacetoinen_US
dc.subjectbacterial hemoglobinen_US
dc.subjectbutanediolen_US
dc.subjectEnterobacter aerogenesen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.titleEnhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin geneen_US
dc.typeArticleen_US

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