Detection and characterization of two phytoplasma lineages on cucumber (Cucumis sativus L.) with same symptomatology based on virtual RFLP and nucleotide sequence analysis of 16S rDNA

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dc.authorscopusid15830471300
dc.authorscopusid57189333203
dc.authorscopusid13611411000
dc.contributor.authorUsta M.
dc.contributor.authorGüller A.
dc.contributor.authorSipahioğlu H.M.
dc.date.accessioned2024-08-04T20:02:17Z
dc.date.available2024-08-04T20:02:17Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPhytoplasma-like symptoms were observed in cucumbers (Cucumis sativus L.) in Van province of Turkey. The major symptoms observed were severe dwarfing, witches’ broom, rosetting, little leaf, and sterility of plants. Genomic DNA of 8 symptomatic and non-symptomatic plant leaves was isolated for the detection of pathogenic DNA. Of the 8 cucumber leaf samples tested by nested polymerase chain reaction (Nested-PCR), the four yielded the expected 1.25-kb DNA fragments when using universal primer pairs R16mF2/R16mR1 and R16F2n/R16R2. Randomly selected two DNA bands were further cloned into a proper plasmid vector. The recombinant plasmid DNA was sequenced bidirectionally. BLAST and virtual restriction fragment length polymorphism (RFLP) analyses of the 16S rDNA sequence revealed the presence of the “Candidatus Phytoplasma solani” (similarity coefficient 1.00) (GenBank accession no: KX977570) in one of the severely symptomatic cucumber samples and the “Candidatus Phytoplasma trifolii” (similarity coefficient 0.98) (GenBank accession no.: KR080212) in the other. The isolates were designated as Van-trifolii and Van-solani isolates, respectively. No significant differences were observed between the two different phytoplasmas’ symptomatology. To the authors’ knowledge, this is the first report of ‘Ca. P. trifolii’ and ‘Ca. P. solani’ in cucumbers in Turkey. © 2017, Centenary University. All rights reserved.en_US
dc.description.sponsorshipThis study was supported by the grant (Project No: FHD-2016-5205) from Research Fund of Yil University.en_US
dc.identifier.doi10.29133/yyutbd.288346
dc.identifier.endpage308en_US
dc.identifier.issn1308-7576
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85032720327en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage299en_US
dc.identifier.urihttps://doi.org/10.29133/yyutbd.288346
dc.identifier.urihttps://hdl.handle.net/11616/91562
dc.identifier.volume27en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCentenary Universityen_US
dc.relation.ispartofYuzuncu Yil University Journal of Agricultural Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCandidatus phytoplasma solanien_US
dc.subjectCandidatus phytoplasma trifoliien_US
dc.subjectCharacterizationen_US
dc.subjectCucumis sativusen_US
dc.subjectDetectionen_US
dc.titleDetection and characterization of two phytoplasma lineages on cucumber (Cucumis sativus L.) with same symptomatology based on virtual RFLP and nucleotide sequence analysis of 16S rDNAen_US
dc.title.alternativeHıyar (Cucumis sativus L.) bitkisinde aynı simptomatolojiye sahip ıki farklı fitoplazma etmeninin 16S rDNA’sının RFLP ve nükleotid dizi analizleri ile teşhisi ve karakterizasyonuen_US
dc.typeArticleen_US

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