The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1? and VEGF

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dc.authorideren, fatih/0000-0001-8126-2413
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridSalva, Emine/0000-0002-1159-5850;
dc.authorwosideren, fatih/AAS-6286-2020
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.authorwosid/AAD-1704-2020
dc.contributor.authorSalva, Emine
dc.contributor.authorTuran, Suna Ozbas
dc.contributor.authorEren, Fatih
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:39:59Z
dc.date.available2024-08-04T20:39:59Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractRNA interference (RNAi) holds considerable promise as a novel therapeutic strategy in the silencing of disease-causing genes. The development of effective delivery systems is important for the use of small interfering RNA (siRNA) as therapy. In the present study, we investigated the effect on breast cancer cell lines and the co-delivery of liposomes containing siHIF1-alpha and siVEGF. In order to achieve the co-delivery of siHIF1-alpha and siVEGF and to obtain lower cytotoxicity, higher transfection and silencing efficiency, in this study, we used chitosan-coated liposomal formulation as the siRNA delivery system. The obtained particle size and zeta potential values show that the chitosan coating process is an effective parameter for particle size and the zeta potential of liposomes. The liposome formulations loaded with siHIF1-alpha and siVEGF showed good stability and protected siRNA from serum degradation after 24-h of incubation. The expression level of VEGF mRNA was markedly suppressed in MCF-7 and MDA-MB435 cells transfected with chitosan-coated liposomes containing siHIF1-alpha and VEGF siRNA, respectively (95% and 94%). In vitro co-delivery of siVEGF and siHIF1-alpha using chitosan-coated liposome significantly inhibited VEGF (89%) and the HIF1-alpha (62%) protein expression when compared to other liposome formulations in the MDA-MB435 cell. The co-delivery of siVEGF and siHIF1-alpha was greatly enhanced in the vitro gene silencing efficiency. In addition, chitosan-coated liposomes showed 96% cell viability. Considering the role of VEGF and HIF1-alpha in breast cancer, siRNA-based therapies with chitosan coated liposomes may have some promises in cancer therapy. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipNovartisen_US
dc.description.sponsorshipThis study was financially supported by grants from the Novartis. We would like to thank Prof. Dr. K.S. Korkmaz for kindly providing the MCF-7 cell line.en_US
dc.identifier.doi10.1016/j.ijpharm.2014.10.065
dc.identifier.endpage154en_US
dc.identifier.issn0378-5173
dc.identifier.issn1873-3476
dc.identifier.issue1en_US
dc.identifier.pmid25445537en_US
dc.identifier.scopus2-s2.0-84918583496en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage147en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijpharm.2014.10.065
dc.identifier.urihttps://hdl.handle.net/11616/96634
dc.identifier.volume478en_US
dc.identifier.wosWOS:000348621100018en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofInternational Journal of Pharmaceuticsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectsiRNAen_US
dc.subjectChitosanen_US
dc.subjectLiposomeen_US
dc.subjectVEGFen_US
dc.subjectHIF-1 alphaen_US
dc.subjectBreast canceren_US
dc.titleThe enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1? and VEGFen_US
dc.typeArticleen_US

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