Protective effect of low dose of melatonin against cholestatic oxidative stress after common bile duct ligation in rats

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dc.authoridPolat, Alaadin/0000-0002-6920-3856
dc.authoridEşrefoğlu, Mukaddes/0000-0003-3380-1480
dc.authoridGül, Mehmet/0000-0002-1374-0783
dc.authorwosidPolat, Alaadin/AAA-7171-2021
dc.authorwosidEşrefoğlu, Mukaddes/JWA-4590-2024
dc.authorwosidGül, Mehmet/ABI-6336-2020
dc.contributor.authorEsrefoglu, Mukaddes
dc.contributor.authorGul, Mehmet
dc.contributor.authorEmre, Memet Hanifi
dc.contributor.authorPolat, Alaattin
dc.contributor.authorSelimoglu, Mukadder Ayse
dc.date.accessioned2024-08-04T20:13:50Z
dc.date.available2024-08-04T20:13:50Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAIM: To investigate the role of oxidative injury and the effect of exogenous melatonin administration on liver damage induced by bile duct ligation (BDL), and second, to evaluate the role of nitric oxide (NO), a free oxygen radical, in oxidative injury. METHODS: Thirty-two Sprague-Dawley rats were assigned to four groups: sham operation (SO), BDL, BDL+melatonin, and BDL+vehicle. Cholestasis was achieved by double ligature of the common bile duct. Melatonin was injected intraperitoneally 500 mu g/(kg.d) for 8 d. Hepatic oxidative stress markers were evaluated by changes in the amount of lipid peroxides, measured as malondialdehyde (MDA), and reduced GSH. Total nitrite (NO(X)) concentrations were determined in hepatic homogenates. Histopathological examination was performed using a histological scoring system. RESULTS: The histopathological changes including portal inflammation, necrosis, apoptosis, focal inflammation and fibrosis were severe in the BDL and BDL+vehicle groups. There were numerous large areas of coagulation necrosis. Histological Activity Index scores of these groups were significantly higher than that of the SO group. Treatment with melatonin reduced these alterations significantly. The degree of necro-inflammation and fibrosis showed significant difference between the BDL and BDL+melatonin groups. BDL was accompanied by a significant increase in MDA and NO(X), and a significant decrease in GSH levels. Mean +/- SE values of MDA, GSH and NO(X) levels of SO group were 147.47 +/- 6.69, 0.88 +/- 0.33 mu mol/g and 180.70 +/- 6.58 nm/g, respectively. The values of BDL group were 200.14 +/- 21.30, 0.65 +/- 0.02 mu mol/g, and 400.46 +/- 48.89 nm/g, respectively, whereas the values of BDL+melatonin group were 115.93 +/- 6.8, 0.74 +/- 0.02 mu mol/g, and 290.38 +/- 32.32 nm/g, respectively. Melatonin treatment was associated with a significant recovery of MDA, GSH and NO(X) levels. CONCLUSION: We have concluded that oxidative stress is associated with the pathogenesis of cholestatic liver damage and NO contributes to oxidative damage. Melatonin, even at low dose, is an efficient agent in reducing negative parameters of cholestasis. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.en_US
dc.identifier.endpage1956en_US
dc.identifier.issn1007-9327
dc.identifier.issue13en_US
dc.identifier.pmid15800985en_US
dc.identifier.scopus2-s2.0-17144389914en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1951en_US
dc.identifier.urihttps://hdl.handle.net/11616/93877
dc.identifier.volume11en_US
dc.identifier.wosWOS:000208102500010en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBaishideng Publ Grp Co Ltden_US
dc.relation.ispartofWorld Journal of Gastroenterologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCholestasisen_US
dc.subjectMelatoninen_US
dc.subjectOxidative stressen_US
dc.subjectFree radicalsen_US
dc.subjectHepatic injuryen_US
dc.titleProtective effect of low dose of melatonin against cholestatic oxidative stress after common bile duct ligation in ratsen_US
dc.typeArticleen_US

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