Comparison of DiOC6(3) uptake and annexin V labeling for quantification of apoptosis in leukemia cells and non-malignant T lymphocytes from children

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dc.authorscopusid6603697741
dc.authorscopusid7004209687
dc.authorscopusid7102641563
dc.authorscopusid7006234945
dc.contributor.authorÖzgen U.
dc.contributor.authorSavaşan S.
dc.contributor.authorBuck S.
dc.contributor.authorRavindranath Y.
dc.date.accessioned2024-08-04T19:59:17Z
dc.date.available2024-08-04T19:59:17Z
dc.date.issued2000
dc.departmentİnönü Üniversitesien_US
dc.description.abstractEarly during apoptosis, there is a reduction in mitochondrial transmembrane potential (MTP) and externalization of phosphatidylserine (PS) in cell membrane prior to eventual cell death. Flow cytometric detection techniques targeting these changes, reduction of DiOC6(3) uptake upon the collapse of MTP and annexin V binding to PS have been successfully used to detect apoptotic cells. These methods have given comparable results when cell lines were used. We compared the two different techniques, DiOC6(3) uptake and Annexin V-propidium iodide co-labeling in the quantification of cytarabine, vincristine and daunorubicin induced apoptosis on three leukemia cell lines (HL-60, CEM, U937), and bone marrow blasts from 26 children with acute myeloid leukemia, 14 with T cell acute lymphoblastic leukemia. Anti- Fas-induced apoptosis in culture-grown peripheral blood T lymphocytes on 18 samples from 9 children with non-malignant conditions were also studied by these techniques. Our results showed that there is a correlation (P < 0.05) between the apoptosis rates measured by these two techniques for drug- induced apoptosis in myeloid and lymphoid blasts, and for anti-Fas mAb- induced apoptosis in T lymphocytes. This data suggests that reduction of the MTP and PS externalization may be common to many apoptotic pathways and techniques targeting either of these changes may be used in quantification of apoptosis in different clinical samples. (C) 2000 Wiley-Liss, Inc.en_US
dc.identifier.doi10.1002/(SICI)1097-0320(20000215)42:1<74
dc.identifier.endpage78en_US
dc.identifier.issn0196-4763
dc.identifier.issue1en_US
dc.identifier.pmid10679746en_US
dc.identifier.scopus2-s2.0-1642633618en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage74en_US
dc.identifier.urihttps://doi.org/10.1002/(SICI)1097-0320(20000215)42:1<74
dc.identifier.urihttps://hdl.handle.net/11616/90532
dc.identifier.volume42en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofCommunications in Clinical Cytometryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnnexin Ven_US
dc.subjectApoptosisen_US
dc.subjectDiOC6(3)en_US
dc.subjectFasen_US
dc.subjectFlow cytometryen_US
dc.subjectLeukemiaen_US
dc.titleComparison of DiOC6(3) uptake and annexin V labeling for quantification of apoptosis in leukemia cells and non-malignant T lymphocytes from childrenen_US
dc.typeArticleen_US

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