Cytotoxic and genotoxic effects of nateglinide on human ovarian, prostate, and colon cancer cell lines

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dc.contributor.authorÖz, Samet
dc.contributor.authorŞekerci, Güldeniz
dc.contributor.authorYüksel, Furkan
dc.contributor.authorTekin, Suat
dc.date.accessioned2024-08-04T19:42:43Z
dc.date.available2024-08-04T19:42:43Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAim: Nateglinide, an oral anti-diabetic medication used to treat type 2 diabetes, activates ATP-dependent potassium channels in pancreatic beta cells and induces insulin secretion. Numerous antidiabetic medicines, particularly metformin, are known to drastically reduce the viability of cancer cells. This study examined the effects of nateglinide on the DNA and viability of human ovarian (A2780), prostate (LNCaP), and colon (Caco-2) cancer cells. Materials and Methods: Initially in the study, 1, 10, 100, and 1000 µM doses of nateglinide were administered for 24 hours to A2780, LNCaP, and Caco-2 cells. The 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was used to measure cell viability. Using Graphpad Prism 8, the inhibitory logarithmic concentration values (LogIC50) of nateglinide in A2780, LNCaP, and Caco-2 cells were computed based on the results of the MTT experiment. These doses were applied to A2780, LNCaP, and Caco-2 cells for the Comet assay. The Bonferroni-corrected Mann–Whitney U test was used to compare groups, and a value of p<0.05 was considered statistically significant. Results: In A2780 and LNCaP cell lines, only 1000 µM nateglinide concentration de creased cell viability (p<0.05), whereas in Caco-2 cells, all concentrations except 1 µM reduced cell viability (p<0.05). The Comet assay indicated that nateglinide produced DNA damage by increasing the tail lengths and tail moments of A2780, LNCaP, and Caco-2 cells (p<0.05) and reducing the head diameters (p<0.05). Conclusion: According to the findings of this study, nateglinide has cytotoxic effects on human ovarian, prostate and colon cancer cell lines and may possess anticancer properties.en_US
dc.identifier.doi10.5455/annalsmedres.2023.02.062
dc.identifier.endpage512en_US
dc.identifier.issn2636-7688
dc.identifier.issue4en_US
dc.identifier.startpage508en_US
dc.identifier.trdizinid1165366en_US
dc.identifier.urihttps://doi.org/10.5455/annalsmedres.2023.02.062
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1165366
dc.identifier.urihttps://hdl.handle.net/11616/88616
dc.identifier.volume30en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofAnnals of Medical Researchen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleCytotoxic and genotoxic effects of nateglinide on human ovarian, prostate, and colon cancer cell linesen_US
dc.typeArticleen_US

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