Use of transcription-based amplification and enzyme immunoassay methods to investigate possible Chlamydia trachomatis infections in women with genital complaints

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dc.authorscopusid6701818265
dc.authorscopusid7003291643
dc.authorscopusid57195695569
dc.authorscopusid6602612326
dc.contributor.authorBulut Y.
dc.contributor.authorDurmaz B.
dc.contributor.authorDurmaz R.
dc.contributor.authorOtlu B.
dc.date.accessioned2024-08-04T19:59:26Z
dc.date.available2024-08-04T19:59:26Z
dc.date.issued2002
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study was performed to determine the prevalence of Chlamydia trachomatis infections in the female patients with genital complaints, and to compare the transcription mediated amplification assay and enzyme immunoassay methods for the diagnosis of genital C. trachomatis infections. C. trachomatis (Ct) antigens and ribosomal RNAs were researched by enzyme immunoassay (EIA) and transcription-mediated amplification (TMA) assay, respectively in the endocervical swab samples of 90 patients. C. trachomatis IgG and IgM antibodies were also screened in the sera of these subjects, by EIA method. Of 90 patients, 18 (20%) were found to be positive for Ct-rRNA, 12 (13%) for Ct-antigen, 20 (22%) for IgG, 12 (13%) for IgM and 14 (16%) for both IgG and IgM. Among the patients 11 (12%) were found positive for Ct-antigen, Ct-rRNA and Ct-IgM antibodies. According to the TMA results, the sensitivity and specificity of EIA-Ct antigen method were estimated as 67% and 100%, respectively. There was statistically significant difference between TMA positivity and those of two EIA methods. In conclusion, the positive results obtained with EIA are reliable for the diagnosis of genital C. trachomatis infections, however the negative results should be confirmed by TMA.en_US
dc.identifier.endpage39en_US
dc.identifier.issn0374-9096
dc.identifier.issue1en_US
dc.identifier.pmid12476764en_US
dc.identifier.scopus2-s2.0-1842836366en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://hdl.handle.net/11616/90628
dc.identifier.volume36en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isotren_US
dc.relation.ispartofMikrobiyoloji bültenien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbacterial antigenen_US
dc.subjectbacterial RNAen_US
dc.subjectbacterium antibodyen_US
dc.subjectimmunoglobulin Gen_US
dc.subjectimmunoglobulin Men_US
dc.subjectribosome RNAen_US
dc.subjectadolescenten_US
dc.subjectadulten_US
dc.subjectarticleen_US
dc.subjectblooden_US
dc.subjectChlamydia trachomatisen_US
dc.subjectchlamydiasisen_US
dc.subjectcomparative studyen_US
dc.subjectfemaleen_US
dc.subjectgeneticsen_US
dc.subjectgynecologic diseaseen_US
dc.subjecthumanen_US
dc.subjectimmunologyen_US
dc.subjectisolation and purificationen_US
dc.subjectmicrobiologyen_US
dc.subjectprevalenceen_US
dc.subjectsensitivity and specificityen_US
dc.subjectTurkey (republic)en_US
dc.subjectuterine cervixen_US
dc.subjectAdolescenten_US
dc.subjectAdulten_US
dc.subjectAntibodies, Bacterialen_US
dc.subjectAntigens, Bacterialen_US
dc.subjectCervix Uterien_US
dc.subjectChlamydia Infectionsen_US
dc.subjectChlamydia trachomatisen_US
dc.subjectFemaleen_US
dc.subjectGenital Diseases, Femaleen_US
dc.subjectHumansen_US
dc.subjectImmunoglobulin Gen_US
dc.subjectImmunoglobulin Men_US
dc.subjectPrevalenceen_US
dc.subjectRNA, Bacterialen_US
dc.subjectRNA, Ribosomalen_US
dc.subjectSensitivity and Specificityen_US
dc.subjectTurkeyen_US
dc.titleUse of transcription-based amplification and enzyme immunoassay methods to investigate possible Chlamydia trachomatis infections in women with genital complaintsen_US
dc.title.alternativeGenital yakinmasi olan kadinlarda Chlamydia trachomatis enfeksiyonunun, transkripsiyon esasli amplifikasyon ve enzim immünoassay yöntemleri ile araştirilmasi.en_US
dc.typeArticleen_US

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