Synthesis and characterization of PMMA composites activated with starch for immobilization of L-asparaginase

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dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridKoytepe, Suleyman/0000-0002-4788-278X
dc.authorwosidKöytepe, Süleyman/AAA-4168-2021
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.contributor.authorUlu, Ahmet
dc.contributor.authorKoytepe, Suleyman
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:41:51Z
dc.date.available2024-08-04T20:41:51Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPoly (methyl methacrylate) (PMMA)-starch composites were prepared by emulsion polymerization technique for L-asparaginase (L-ASNase) immobilization as highly activated support. The hydroxide groups on the prepared composites offer a very simple, mild and firm combination for enzyme immobilization. The pure PMMA and PMMA-starch composites were characterized as structural, thermal and morphological. PMMA-starch composites were found to have better thermal stability and more hydrophilic character than pure PMMA. L-ASNase was immobilized onto PMMA-starch composites contained the different ratio of starch (1, 3, 5, and 10 wt %). Immobilized L-ASNase showed better performance as compared to the native enzyme in terms of thermal stability and pH. K-m value of immobilized enzyme decreased approximately eightfold compared with the native enzyme. In addition to, immobilized L-ASNase was found to retain 60% of activity after 1-month storage period at 4 degrees C. Therefore, PMMA-starch composites can be provided more advantageous in terms of enzymatic affinity, thermal, pH and storage stability as L-ASNase immobilization matrix. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43421.en_US
dc.description.sponsorshipInonu University Scientific Research Projects Unit [BAP 2013/37]en_US
dc.description.sponsorshipThis work was partially supported by The Inonu University Scientific Research Projects Unit (Grant No. BAP 2013/37).en_US
dc.identifier.doi10.1002/app.43421
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue19en_US
dc.identifier.scopus2-s2.0-84976241880en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/app.43421
dc.identifier.urihttps://hdl.handle.net/11616/97388
dc.identifier.volume133en_US
dc.identifier.wosWOS:000370445800022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Applied Polymer Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbiodegradableen_US
dc.subjectbiomedical applicationsen_US
dc.subjectbiopolymers and renewable polymersen_US
dc.subjectemulsion polymerizationen_US
dc.titleSynthesis and characterization of PMMA composites activated with starch for immobilization of L-asparaginaseen_US
dc.typeArticleen_US

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