Toxin-neutralizing antibodies elicited by naturally acquired cutaneous anthrax are elevated following severe disease and appear to target conformational epitopes

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dc.authoridDoganay, Mehmet/0000-0003-3922-4901
dc.authoridKayabas, Uner/0000-0002-5323-0796
dc.authoridBoons, Geert-Jan/0000-0003-3111-5954
dc.authorwosidDoganay, Mehmet/G-4250-2019
dc.authorwosidKayabas, Uner/JRX-1616-2023
dc.authorwosidBoons, Geert-Jan/J-3211-2016
dc.contributor.authorDumas, Eric K.
dc.contributor.authorDemiraslan, Hayati
dc.contributor.authorIngram, Rebecca J.
dc.contributor.authorSparks, Rebecca M.
dc.contributor.authorMuns, Emily
dc.contributor.authorZamora, Adriana
dc.contributor.authorLarabee, Jason
dc.date.accessioned2024-08-04T20:47:14Z
dc.date.available2024-08-04T20:47:14Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractUnderstanding immune responses to native antigens in response to natural infections can lead to improved approaches to vaccination. This study sought to characterize the humoral immune response to anthrax toxin components, capsule and spore antigens in individuals (n = 46) from the Kayseri and Malatya regions of Turkey who had recovered from mild or severe forms of cutaneous anthrax infection, compared to regional healthy controls (n = 20). IgG antibodies to each toxin component, the poly-gamma- D-glutamic acid capsule, the Bacillus collagen-like protein of anthracis (BclA) spore antigen, and the spore carbohydrate anthrose, were detected in the cases, with anthrax toxin neutralization and responses to Protective Antigen (PA) and Lethal Factor (LF) being higher following severe forms of the disease. Significant correlative relationships among responses to PA, LF, Edema Factor (EF) and capsule were observed among the cases. Though some regional control sera exhibited binding to a subset of the tested antigens, these samples did not neutralize anthrax toxins and lacked correlative relationships among antigen binding specificities observed in the cases. Comparison of serum binding to overlapping decapeptides covering the entire length of PA, LF and EF proteins in 26 cases compared to 8 regional controls revealed that anthrax toxin-neutralizing antibody responses elicited following natural cutaneous anthrax infection are directed to conformational epitopes. These studies support the concept of vaccination approaches that preserve conformational epitopes.en_US
dc.description.sponsorshipNational Institutes of Health/National Institute of Allergy and Infectious Diseases [5U19AI062629-09, 5U19AI062629-10, 5U19AI062629-11, 3T32AI007633]; National Institutes of Health/National Institute of General Medical Sciences [3P30GM103510, 5U54GM104938]; Oklahoma Medical Research Foundation J. Donald and Patricia Capra Fellowship; Oklahoma Medical Research Foundation Lou C. Kerr Chair in Biomedical Researchen_US
dc.description.sponsorshipNational Institutes of Health/National Institute of Allergy and Infectious Diseases: 5U19AI062629-09, JAJ, ADF, https://projectreporter.nih.gov/project_info_description.cfm?aid=8320322 & icde=0; 5U19AI062629-10, JAJ, ADF, https://projectreporter.nih.gov/project_ info_description.cfm?aid=8526353 & icde=0;5U19AI062629-11, JAJ, ADF, https://projectreporter.nih.gov/project_info_description.cfm?aid=8708258&icde=0 National Institutes of Health/National Institute of Allergy and Infectious Diseases: 3T32AI007633 LG; https://projectreporter.nih.gov/project_info_details.cfm? aid=9706714&icde=36340871 National Institutes of Health/National Institute of General Medical Sciences: 3P30GM103510 JAJ; https://projectreporter.nih.gov/project_info_description.cfm?aid=9317706&icde=36340932 National Institutes of Health/National Institute of General Medical Sciences: 5U54GM104938 JAJ; https://projectreporter.nih.gov/project_info_description.cfm?aid=9755446&icde=0 Oklahoma Medical Research Foundation J. Donald and Patricia Capra Fellowship: no grant number JAJ; no URL Oklahoma Medical Research Foundation Lou C. Kerr Chair in Biomedical Research: no grant number JAJ; no URL The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The opinions and assertions contained herein are private views of the authors and are not to be construed as official or as reflecting the views of the NIH or other government agencies.en_US
dc.identifier.doi10.1371/journal.pone.0230782
dc.identifier.issn1932-6203
dc.identifier.issue4en_US
dc.identifier.pmid32294093en_US
dc.identifier.scopus2-s2.0-85083292309en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0230782
dc.identifier.urihttps://hdl.handle.net/11616/99250
dc.identifier.volume15en_US
dc.identifier.wosWOS:000536003500012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLinked-Immunosorbent-Assayen_US
dc.subjectBacillus-Anthracisen_US
dc.subjectProtective Antigenen_US
dc.subjectInhalational Anthraxen_US
dc.subjectRhesus Macaquesen_US
dc.subjectGuinea-Pigsen_US
dc.subjectFactor-Ien_US
dc.subjectVaccineen_US
dc.subjectReceptoren_US
dc.subjectInfectionen_US
dc.titleToxin-neutralizing antibodies elicited by naturally acquired cutaneous anthrax are elevated following severe disease and appear to target conformational epitopesen_US
dc.typeArticleen_US

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