Distribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkey

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dc.authoridALP, ALPASLAN/0000-0001-7856-7590
dc.authoridRASTOGI, NALIN/0000-0002-7199-7747
dc.authoridGünal, Selami/0000-0002-4752-5176
dc.authoridAlbay, Ali/0000-0003-2778-8910
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidALP, ALPASLAN/I-8366-2013
dc.authorwosidRASTOGI, NALIN/AAP-1958-2020
dc.authorwosidGünal, Selami/AAA-7246-2021
dc.authorwosidceyhan, ismail/HKW-0007-2023
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.contributor.authorKisa, Ozgul
dc.contributor.authorTarhan, Gulnur
dc.contributor.authorGunal, Selami
dc.contributor.authorAlbay, Ali
dc.contributor.authorDurmaz, Riza
dc.contributor.authorSaribas, Zeynep
dc.contributor.authorZozio, Thierry
dc.date.accessioned2024-08-04T20:35:42Z
dc.date.available2024-08-04T20:35:42Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Investigation of genetic heterogeneity and spoligotype-defined lineages of drug-resistant Mycobacterium tuberculosis clinical isolates collected during a three-year period in two university hospitals and National Tuberculosis Reference and Research Laboratory in Ankara, Turkey. Methods and Findings: A total of 95 drug-resistant M. tuberculosis isolates collected from three different centers were included in this study. Susceptibility testing of the isolates to four major antituberculous drugs was performed using proportion method on Lowenstein-Jensen medium and BACTEC 460-TB system. All clinical isolates were typed by using spoligotyping and IS6110-restriction fragment length polymorphism (RFLP) methods. Seventy-three of the 95 (76.8%) drug resistant M. tuberculosis isolates were isoniazid-resistant, 45 (47.4%) were rifampicin-resistant, 32 (33.7%) were streptomycin-resistant and 31 (32.6%) were ethambutol-resistant. The proportion of multidrug-resistant isolates (MDR) was 42.1%. By using spoligotyping, 35 distinct patterns were observed; 75 clinical isolates were grouped in 15 clusters (clustering rate of 79%) and 20 isolates displayed unique patterns. Five of these 20 unique patterns corresponded to orphan patterns in the SITVIT2 database, while 4 shared types containing 8 isolates were newly created. The most prevalent M. tuberculosis lineages were: Haarlem (23/95, 24.2%), ill-defined T superfamily (22/95, 23.2%), the Turkey family (19/95, 20%; previously designated as LAM7-TUR), Beijing (6/95, 6.3%), and Latin-America & Mediterranean (LAM, 5/95 or 5.3%), followed by Manu (3/95, 3.2%) and S (1/95, 1%) lineages. Four of the six Beijing family isolates (66.7%) were MDR. A combination of IS6110-RFLP and spoligotyping reduced the clustering rate from 79% to 11.5% among the drug resistant isolates. Conclusions: The results obtained showed that ill-defined T, Haarlem, the Turkey family (previously designated as LAM7-TUR family with high phylogeographical specifity for Turkey), Beijing and LAM were predominant lineages observed in almost 80% of the drug-Resistant M. tuberculosis complex clinical isolates in Ankara, Turkey.en_US
dc.identifier.doi10.1371/journal.pone.0030331
dc.identifier.issn1932-6203
dc.identifier.issue1en_US
dc.identifier.pmid22279583en_US
dc.identifier.scopus2-s2.0-84855930679en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0030331
dc.identifier.urihttps://hdl.handle.net/11616/95539
dc.identifier.volume7en_US
dc.identifier.wosWOS:000299771900060en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenetic Diversityen_US
dc.subjectGlobal Distributionen_US
dc.subjectEastern Turkeyen_US
dc.subjectEpidemiologyen_US
dc.subjectStrainsen_US
dc.subjectFamiliesen_US
dc.subjectDatabaseen_US
dc.titleDistribution of Spoligotyping Defined Genotypic Lineages among Drug-Resistant Mycobacterium tuberculosis Complex Clinical Isolates in Ankara, Turkeyen_US
dc.typeArticleen_US

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