Membrane permeabilization of gram-negative bacteria with a potassium phosphate/hexane aqueous phase system for the release of L-asparaginase: an enzyme used in cancer therapy

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dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridUÇKUN, Miraç/0000-0002-9018-8515
dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidMiraç, Uçkun/ABG-5824-2021
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidUÇKUN, Miraç/ABC-4209-2021
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorGeckil, H
dc.contributor.authorAtes, B
dc.contributor.authorGencer, S
dc.contributor.authorUckun, M
dc.contributor.authorYilmaz, I
dc.date.accessioned2024-08-04T20:32:15Z
dc.date.available2024-08-04T20:32:15Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractA fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from two distinctly related gram-negative bacteria, Enterobacter aerogenes and Pseudomonas aeruginosa, is presented. As the method uses inexpensive organic solvent hexane and an aqueous salt solution, it is also highly cost-effective in comparison with the currently available techniques used for the release of this enzyme. As hexane is a highly water immiscible organic solvent, it can be removed easily from the top of the aqueous phase by a simple evaporation. Also, various organic solvents and other membrane partitioning compounds were compared for their efficiency on L-asparaginase/protein release. The degree to which the enzyme was released was different for two bacteria, suggesting that they possess different permeability characteristics. The most efficient enzyme release from both bacteria was determined to be in 50 mM potassium phosphate with 1% hexane. Enzyme recoveries up to three-fold with respect to sonication have been achieved with this system. (C) 2004 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.procbio.2004.01.033
dc.identifier.endpage579en_US
dc.identifier.issn1359-5113
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-7444233196en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage573en_US
dc.identifier.urihttps://doi.org/10.1016/j.procbio.2004.01.033
dc.identifier.urihttps://hdl.handle.net/11616/94954
dc.identifier.volume40en_US
dc.identifier.wosWOS:000225235400010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectL-asparaginaseen_US
dc.subjectperiplasmic enzymesen_US
dc.subjectmembrane permeabilizationen_US
dc.subjectorganic solventsen_US
dc.subjecthexaneen_US
dc.titleMembrane permeabilization of gram-negative bacteria with a potassium phosphate/hexane aqueous phase system for the release of L-asparaginase: an enzyme used in cancer therapyen_US
dc.typeArticleen_US

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