Metisiline dirençli stafilokoklarda vankomisine karşı azalmış duyarlılığın araştırılması
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Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Staphylococcus aureus, tüm dünyada toplum ve hastane kaynaklı enfeksiyonlara yol açan en önemli etkenlerden biridir. VRSA'da bakteriler vankomisine karşı çok yüksek MİK değerlerine sahip olduğundan, rutin duyarlılık testlerinde tespit edilebilmektedir. VISA/hVISA suşlarının ise laboratuvarlarda uygulanan rutin duyarlılık testleriyle saptanması güçtür. Çalışmamızda retrospektif olarak Ocak 2008 - Ağustos 2013 tarihleri arasında İnönü Üniversitesi Turgut Özal Tıp Merkezi Mikrobiyoloji Laboratuvarına gönderilmiş çeşitli örneklerden izole edilip -80 °C de stoklanmış 52 adet MRSA suşu kullanıldı. Bu suşlarda agar tarama, standart E test, makro E-test, buyyon mikrodilüsyon ve PAP-AUC yöntemleriyle VISA ve hVISA izolatlarının sıklığının araştırılması amaçlanmıştır. Bu örnekler öncelikle mecA, nuc ve 16s genleri açısından moleküler olarak test edildi. Tüm suşlar mecA, nuc ve 16s genlerinin her üçü için pozitifti. Bu suşlara vankomisin agar tarama, vankomisin mikrodilüsyon ve standart E-testleri uygulandı. Agar taramada suşlardan sekiz tanesi 24. saatte, bir tanesi 48. saatte üreyerek toplamda dokuz suş üredi. Dokuz suşa yapılan PAP-AUC sonuçlarına göre dokuz suşta (% 17,3) hVISA olarak tespit edildi.
Staphylococcus aureus is one of the most important factors that cause the whole world community and hospital-caused infections. They can be detected in routine susceptibility testing due to the fact that bacteria have very high MIC values against vancomycin in VRSA. On the other hand, it is difficult to detect with routine susceptibility testing applied in the laboratory of VISA/hVISA strains. In our study study, 52 MRSA strains were detected to the Inonu University Turgut Ozal Medical Center Microbiology Laboratory between January 2008 and August 2013 as retrospective isolated from different samples and stocked at -80 °C were used. İt was aimed to investigate the frequency of VISA and hVISA isolates with agar screening, standard E-test, macro E-test, broth microdilution and PAP-AUC methods in these strains. These examples were primarily tested as molecular in terms of with regard to genes of mecA, nuc and 16s. All strains were positive for each of three genes, that is, mecA, nuc and 16s. Vancomycin agar screening, vancomycin microdilution and E-test standards were applied to these strains. Eight of strains were grown at 24 hours an done of them was grown at 48 hours and totaly 9 strains were grown. According to PAP-AUC applied to 9 strains it was detected as 17.3% hVISA at 9 strains.
Staphylococcus aureus is one of the most important factors that cause the whole world community and hospital-caused infections. They can be detected in routine susceptibility testing due to the fact that bacteria have very high MIC values against vancomycin in VRSA. On the other hand, it is difficult to detect with routine susceptibility testing applied in the laboratory of VISA/hVISA strains. In our study study, 52 MRSA strains were detected to the Inonu University Turgut Ozal Medical Center Microbiology Laboratory between January 2008 and August 2013 as retrospective isolated from different samples and stocked at -80 °C were used. İt was aimed to investigate the frequency of VISA and hVISA isolates with agar screening, standard E-test, macro E-test, broth microdilution and PAP-AUC methods in these strains. These examples were primarily tested as molecular in terms of with regard to genes of mecA, nuc and 16s. All strains were positive for each of three genes, that is, mecA, nuc and 16s. Vancomycin agar screening, vancomycin microdilution and E-test standards were applied to these strains. Eight of strains were grown at 24 hours an done of them was grown at 48 hours and totaly 9 strains were grown. According to PAP-AUC applied to 9 strains it was detected as 17.3% hVISA at 9 strains.
Açıklama
Anahtar Kelimeler
Mikrobiyoloji, Microbiology
Kaynak
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Künye
Tunç, E. (2014). Metisiline dirençli stafilokoklarda vankomisine karşı azalmış duyarlılığın araştırılması. Yayımlanmış Uzmanlık Tezi, İnönü Üniversitesi, Malatya.
