Evaluation of estrogenic effects of polychlorinated biphenyls and organochlorinated pesticides using immature rat uterotrophic assay

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dc.authoridSandal, Suleyman/0000-0002-8916-3329
dc.authoridYilmaz, Bayram/0000-0002-2674-6535
dc.authoridUSLU, UNAL/0000-0003-3953-7131
dc.authoridCumbul, Alev/0000-0002-9491-8220
dc.authorwosidCUMBUL, Alev/H-3991-2011
dc.authorwosidSandal, Suleyman/AAA-6388-2021
dc.authorwosidYilmaz, Bayram/IXD-3395-2023
dc.authorwosidYILDIZ, SEDAT/AAB-6354-2021
dc.authorwosidUSLU, UNAL/H-1226-2011
dc.contributor.authorUslu, U.
dc.contributor.authorSandal, S.
dc.contributor.authorCumbul, A.
dc.contributor.authorYildiz, S.
dc.contributor.authorAydin, M.
dc.contributor.authorYilmaz, B.
dc.date.accessioned2024-08-04T20:37:36Z
dc.date.available2024-08-04T20:37:36Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, we investigated the effects of polychlorinated biphenyls (PCBs) and organochlorinated pesticides on the serum levels of luteinising hormone (LH), follicle stimulating hormone (FSH) and weights and histomorphometry of uterine tissue in immature female rats using uterotrophic assay. A total of 36 rats were randomly divided into six groups (n = 6 per group) as control, oestradiol (E-2, 100 mu g/kg), PCB 180, Aroclor 1221, endosulfan and mirex at 10 mg/kg dosage. After 3 days of injections (subcutaneous), animals were decapitated and blood samples were collected. Uteri were dissected, weighed out and then fixed in 10% formaldehyde. They were processed for histomorphometry. The serum levels of LH and FSH were determined by enzyme immunoassay. Uterine weight was significantly increased by E-2 and reduced by mirex (p < 0.001 and p < 0.05, respectively). Total volume of uterus was significantly raised by E-2, Aroclor 1221 and endosulfan compared with that of the control group (p < 0.01). The ratio of epithelium was significantly increased by E-2, PCBs and pesticides (p < 0.01). The uterine cavity ratio was decreased by aroclor (p < 0.01), PCB 180 and mirex (p < 0.05). The serum levels of LH did not significantly differ among the groups but the levels of FSH were decreased by PCB 180 and endosulfan (p < 0.05 and p < 0.01, respectively). These findings suggest that PCB 180, Aroclor 1221 and endosulfan may be estrogenic in immature uterotrophic assay.en_US
dc.description.sponsorshipTUBITAK [104-T-240]en_US
dc.description.sponsorshipThis study was supported by TUBITAK (project: 104-T-240).en_US
dc.identifier.doi10.1177/0960327112472999
dc.identifier.endpage482en_US
dc.identifier.issn0960-3271
dc.identifier.issn1477-0903
dc.identifier.issue5en_US
dc.identifier.pmid23515497en_US
dc.identifier.scopus2-s2.0-84878317205en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage476en_US
dc.identifier.urihttps://doi.org/10.1177/0960327112472999
dc.identifier.urihttps://hdl.handle.net/11616/96071
dc.identifier.volume32en_US
dc.identifier.wosWOS:000319223100003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofHuman & Experimental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPCBsen_US
dc.subjectpesticidesen_US
dc.subjectuterotrophic assayen_US
dc.subjectLHen_US
dc.subjectFSHen_US
dc.subjecthistomorphometryen_US
dc.titleEvaluation of estrogenic effects of polychlorinated biphenyls and organochlorinated pesticides using immature rat uterotrophic assayen_US
dc.typeArticleen_US

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