Kisspeptin-10 elicits triphasic cytosolic calcium responses in immortalized GT1-7 GnRH neurones

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dc.authoridSandal, Suleyman/0000-0002-8916-3329
dc.authoridYilmaz, Bayram/0000-0002-2674-6535
dc.authoridOzcan, Mete/0000-0002-5551-4880;
dc.authorwosidSandal, Suleyman/AAA-6388-2021
dc.authorwosidYilmaz, Bayram/IXD-3395-2023
dc.authorwosidOzcan, Mete/V-8758-2018
dc.authorwosidAYAR, Ahmet/I-1379-2014
dc.authorwosidKelestimur, Haluk/V-9392-2018
dc.contributor.authorOzcan, Mete
dc.contributor.authorAlcin, Ergul
dc.contributor.authorAyar, Ahmet
dc.contributor.authorYilmaz, Bayram
dc.contributor.authorSandal, Suleyman
dc.contributor.authorKelestimur, Haluk
dc.date.accessioned2024-08-04T20:32:43Z
dc.date.available2024-08-04T20:32:43Z
dc.date.issued2011
dc.departmentİnönü Üniversitesien_US
dc.description.abstractKisspeptins, which are alternatively called as metastin since they were originally identified as products of metastasis suppressor gene KiSS-1, are the natural ligands for the G protein-coupled receptor 54 (GPR54). Kisspeptins are the most potent activators of hypothalamic-pituitary-gonadal (HPG) axis reported to date. The pulsatile pattern of GnRH release, which results in the intermittent release of gonadotropic hormones from the pituitary, has a critical importance for reproductive function but the factors responsible from this release pattern are not known. Therefore, the pattern of kisspeptin-induced intracellular signaling and the role of PKC in the intracellular signaling cascade were investigated by fluorescence calcium imaging using the immortalized GnRH-secreting GT1-7 hypothalamic neurons. Kisspeptin-10 caused a triphasic change characterized by an initial small increase followed by a significant decrease and increase in intracellular free calcium concentrations ([Ca2+](i)). The changes in [Ca2+](i) were significantly attenuated by pre-treatment with protein kinase C inhibitor. The compatibility of appeared mirrored-patterns of kisspeptin-10-induced changes in [Ca2+](i) concentrations in these neurons and GnRH secretion confirm the importance of intracellular calcium flux downstream from GPR54 through PKC signaling pathway. (C) 2011 Elsevier Ireland Ltd. All rights reserved.en_US
dc.description.sponsorshipTUBITAK [107T825]en_US
dc.description.sponsorshipWe thank Dr. Pamela L. Mellon, Department of Reproductive Medicine, University of California, San Diego, for kindly providing GT1-7 cells. This work was supported by a grant from TUBITAK (Project No. 107T825).en_US
dc.identifier.doi10.1016/j.neulet.2011.01.054
dc.identifier.endpage58en_US
dc.identifier.issn0304-3940
dc.identifier.issn1872-7972
dc.identifier.issue1en_US
dc.identifier.pmid21276835en_US
dc.identifier.scopus2-s2.0-79952002829en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage55en_US
dc.identifier.urihttps://doi.org/10.1016/j.neulet.2011.01.054
dc.identifier.urihttps://hdl.handle.net/11616/95262
dc.identifier.volume492en_US
dc.identifier.wosWOS:000288731800013en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofNeuroscience Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectKisspeptin-10en_US
dc.subjectCalcium signalingen_US
dc.subjectGT1-7 cellsen_US
dc.titleKisspeptin-10 elicits triphasic cytosolic calcium responses in immortalized GT1-7 GnRH neuronesen_US
dc.typeArticleen_US

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