Surface-Engineered TiO2 Film for Enhanced Electrochemical Biosensing and Cell Monitoring

Basit Öğe Kaydı
dc.contributor.authorSanli, Serdar
dc.contributor.authorTabak, Eray
dc.contributor.authorKilinc, Necmettin
dc.contributor.authorKosemen, Arif
dc.contributor.authorErginer, Merve
dc.contributor.authorOzturk, Sadullah
dc.contributor.authorBarlas, Firat Baris
dc.date.accessioned2026-04-04T13:37:45Z
dc.date.available2026-04-04T13:37:45Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractIn vitro cell density measuring studies are mostly based on colorimetric methods; however, these approaches are limited to endpoint measurements rather than continuous data. To obtain more sensitive and continuous data, real-time monitoring of cell density is essential, which requires the development of surfaces with optimal physicochemical properties. TiO2 nanoporous structures are promising due to their favorable electrical properties, simple synthesis, unique porosity, biocompatibility, and stability. This study examined TiO2 films deposited onto titanium plates and screen-printed electrodes, characterized by scanning electron microscopy (SEM) and electrochemical techniques. HeLa and A549 cell proliferation on TiO2 was assessed and compared to polystyrene. Cell adhesion was evaluated via DAPI staining, fluorescence microscopy, and SEM. Electrochemical analyses (CV and EIS) were conducted on TiO2-coated electrodes. Results showed comparable proliferation on TiO2 and polystyrene, with effective adhesion confirmed by SEM. Electrochemical data demonstrated high sensitivity in detecting cellular differences, with detection limits of 150 cells for A549 and 107 for HeLa. These findings highlight TiO2 nanoporous structures as promising candidates for cell-based biosensor platforms.
dc.description.sponsorshipInonu University Scientific Research Project [FCD-2022-2864]
dc.description.sponsorshipThe authors extend their gratitude to Inonu University Scientific Research Project (FCD-2022-2864) for providing financial support. During the preparation of this work, the authors used ChatGPT-4.0 in order to assist with language editing and content generation. After using this tool, the authors thoroughly reviewed and edited the content as needed and take full responsibility for the final version of the published article.
dc.identifier.doi10.1002/anse.202500072
dc.identifier.issn2629-2742
dc.identifier.issue1
dc.identifier.orcid0000-0002-9048-287X
dc.identifier.orcid0000-0002-4851-3062
dc.identifier.orcid0000-0001-6401-686X
dc.identifier.scopus2-s2.0-105014108022
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.1002/anse.202500072
dc.identifier.urihttps://hdl.handle.net/11616/110012
dc.identifier.volume6
dc.identifier.wosWOS:001556492100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofAnalysis & Sensing
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectbiosensors
dc.subjectcell proliferation
dc.subjectscreen-printed electrodes
dc.subjecttiO2 nanoporous
dc.titleSurface-Engineered TiO2 Film for Enhanced Electrochemical Biosensing and Cell Monitoring
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu