Detection and genotyping of Epstein-Barr virus by polymerase chain reaction in tissues obtained from cases with Hodgkin's disease in Turkey

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dc.authoridÖZERCAN, ibrahim Hanifi/0000-0002-8781-8838
dc.authoridkoroglu, mehmet/0000-0001-8101-1104
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidÖZERCAN, ibrahim Hanifi/W-7883-2018
dc.authorwosidkoroglu, mehmet/HTN-0171-2023
dc.authorwosidAtik, Emre/HJG-5889-2022
dc.contributor.authorDurmaz, R
dc.contributor.authorAydin, A
dc.contributor.authorKöroglu, M
dc.contributor.authorAker, H
dc.contributor.authorÖzercan, IH
dc.contributor.authorAtik, E
dc.contributor.authorArici, S
dc.date.accessioned2024-08-04T20:11:59Z
dc.date.available2024-08-04T20:11:59Z
dc.date.issued1998
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn order to determine the positivity rare and genotype of Epstein-Barr virus (EBV) in cases with Hodgkin's disease (HD) in Turkey, 40 tissue specimens from HD patients were analysed. Ten non-lymphoid tissue samples from individuals without any evidence for lymphoma were used as controls. The cases with HD included 33 males and 7 females with a mean age of 28 years. Nodular sclerosis was the most prevalent histological subtype (16/40) followed by mixed cellularity (10/40), lymphocyte predominance (9/40), and lymphocyte depletion (5/40). After histopathological evaluation, deparafinisation and lysis of the specimens, one-stage polymerase chain reaction (PCR) and two-stage (nested) PCR assays were performed with the primers common for both EBV genotypes and the primers specific for EBV types 1 and 2, respectively. EBV DNA was detected in 22 of 40 (55%) cases with HD and in 1 of 10 (10%) control specimens. The distribution of EBV DNA positivity according to the histological subtypes was as follows: 10 of 16 (62.5%) for nodular sclerosis, 3 of 5 (60%) for lymphocyte depletion, 5 of 9 (55.6%) for lymphocyte predominance, and 4 of 10 (40%) for mixed cellularity. Although most of the HD patients were males of 15 - 34 years of age, there were no significant differences between EBV positivities obtained From different sex and age groups. The rates of EBV genotypes were 82% for type 1, 9% for type 2, and 9% for both types, respectively.en_US
dc.identifier.endpage381en_US
dc.identifier.issn0001-723X
dc.identifier.issue6en_US
dc.identifier.pmid10358743en_US
dc.identifier.scopus2-s2.0-0032416784en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage375en_US
dc.identifier.urihttps://hdl.handle.net/11616/93134
dc.identifier.volume42en_US
dc.identifier.wosWOS:000079531900004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSlovak Academic Press Ltden_US
dc.relation.ispartofActa Virologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEpstein-Barr virusen_US
dc.subjectvirus genotypeen_US
dc.subjectHodgkin's diseaseen_US
dc.subjectpolymerase chain reactionen_US
dc.titleDetection and genotyping of Epstein-Barr virus by polymerase chain reaction in tissues obtained from cases with Hodgkin's disease in Turkeyen_US
dc.typeArticleen_US

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