Synthesis of PEITC-loaded gold nanoparticles and evaluation of the hepatoprotective effect on CCl4-induced damage through Nrf2 pathway

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dc.authoridSatilmis, Basri/0000-0002-2538-5774
dc.authorwosidSatilmis, Basri/JBR-9078-2023
dc.contributor.authorUyumlu, Ayse Burcin
dc.contributor.authorYilmaz, Hatice Caglar
dc.contributor.authorSatilmis, Basri
dc.contributor.authorErdemoglu, Sema
dc.date.accessioned2024-08-04T20:53:35Z
dc.date.available2024-08-04T20:53:35Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjectives: The purpose of the study was to prepare an effective and new drug delivery system for enhancing the stability of Phenethyl isothiocyanate (PEITC), and its hepatoprotective effect in the carbon tetrachloride (CCl4)-induced damage in hepatocellular carcinoma G2 (HepG2) cell line via nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway.Methods: Gold nanoparticles were synthesized and then characterized by XRD, SEM, SEM-EDX analysis, hydrodynamic diameter and zeta potential measurements. 1.0024 mM PEITC, a naturally occurring isothiocyanate, an active ingredient was loaded onto the characterized AuNPs. The cytotoxicity test of PEITC-AuNP and effects on ALT, AST, Nrf2 levels and total antioxidant capacity (TAC) of CCI4-induced HepG2 cells were investigated.Results: PEITC-AuNPs and PEITC decreased ALT and AST levels (p < 0.05). This reduction was greater with PEITC-AuNPs. PEITC-AuNPs increased Nrf2 level but it was nonsignificantly (p > 0.05). PEITC didn't increase the Nrf2 level in CCI4-induced HepG2 cells. TAC of both PEITC-AuNPs and PEITC administration increased significantly compared with CCl4 group (p < 0.05). But PEITC-AuNPs enhanced the TAC level higher than PEITC significantly (p < 0.05).Conclusions: PEITC-AuNPs were more effective than PEITC which resulted in more hepatoprotective and antioxidant effects via Nrf2 activation against CCl4-induced liver injury in HepG2 cells.en_US
dc.description.sponsorshipScientific Research Projects Unit of Inoenue University [2020-2039]en_US
dc.description.sponsorshipThis study was funded by the Scientific Research Projects Unit of Inoenue University for funding[Grant No. 2020-2039].en_US
dc.identifier.doi10.1515/tjb-2022-0208
dc.identifier.endpage31en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85152008665en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage26en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2022-0208
dc.identifier.urihttps://hdl.handle.net/11616/101280
dc.identifier.volume48en_US
dc.identifier.wosWOS:000906428100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCCl4en_US
dc.subjectgold nanoparticleen_US
dc.subjectHepG2 cellen_US
dc.subjectNrf2en_US
dc.subjectphenethyl isothiocyanateen_US
dc.titleSynthesis of PEITC-loaded gold nanoparticles and evaluation of the hepatoprotective effect on CCl4-induced damage through Nrf2 pathwayen_US
dc.typeArticleen_US

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