Beyin nöroinflamasyon modelinde resveratrolün oksidatif ve histopatolojik değişiklikler ile SIRT1 geni ifadesi üzerine etkileri
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Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada sıçanlarda LPS-indüklü nöroinflamasyon modeli ile oluşturulan nörodejenerasyonda, resveratrolün ve EX-527'nin beyinde moleküler genetik, biyokimyasal, bilişsel ve histopatolojik parametreler üzerinden var ise nöroprotektif etkilerinin incelenmesi amaçlanmıştır. Materyal ve Metot: Araştırmamızda 55 adet Wistar Albino cinsi erkek sıçan kullanıldı. Sıçanların beyin dokusundan; SIRT1 geninin ifadesi, biyokimyasal analizler ve histopatolojik incelemeler yapıldı. Sıçanların bilişsel incelemeleri Morris su labirenti deneyi ile yapıldı. Bulgular: Araştırmamızda; Res ve Res+LPS grubundaki beyin MDA seviyelerinin, K, LPS ve EX-527+LPS grubuna göre arttığı tespit edildi (p<0.05). EX-527+LPS grubu beyin SOD aktivitesindeki artış, K ve LPS grubuna göre anlamlıyken (p<0.05), Res ve Res+LPS grubu SOD aktivitelerindeki azalma K grubuna göre anlamlı bulundu (p<0.05). EX-527+LPS grubu beyin SIRT1 gen ifadesindeki azalma diğer tüm gruplarla karşılaştırıldığında anlamlı gözlendi (p<0.05). Res+LPS grubunun SIRT1 gen ifadesi K grubuna göre anlamlı bir artış gösterdi (p<0.05). Histopatolojik veriler incelendiğinde; K ve Res gruplarının beyin kesitlerinin normal morfolojik özellik gösterdiği, LPS grubunda, nöronların dejeneratif değişiklik gösterdiği, Res+LPS grubundaki dejenere nöron yoğunluğunun, LPS grubuna göre belirgin şekilde azaldığı, EX-527+LPS grubunda gözlenen nöron dejenerasyonunun, K ve Res gruplarından anlamlı derecede yüksek iken LPS grubundan ise daha hafif düzeyde dejenere nöron yoğunluğuna sahip olduğu tespit edildi (p<0.05). Sonuç: Resveratrol ile SIRT1 gen ifadesinin arttırılmasının, LPS indüklü nörodejenerasyonu önleyebileceği kanaatini taşımaktayız. Anahtar Kelimeler: SIRT1, nöroinflamasyon, nörodejenerasyon resveratrol, LPS, EX-527, sıçan
Aim: The aim of this study is investigating the effects of resveratrol and EX-527 on LPS-induced neuroinflammation rat model brain as molecular genetic, biochemical, cognitive and histopathological and whether there is any neuroprotective activity. Material and Method: This study was carried out with 55 Wistar albino male rats. SIRT1 gene expression, biochemichal analyses and histopathological examination were made in rat brain tissue. Analysis of cognitive effects of disease model was practiced by Morris water maze. Results: In this study, MDA levels of brain tissues in Res and Res+LPS groups were found statistically higher than K, LPS and EX-527+LPS groups (p<0.05). SOD activities in brain tissues of EX-527+LPS were increased in comparision with K and LPS groups statistically (p<0.05) while decreased in Res and Res+LPS groups was significantly compared to K group (p<0.05). Expression of SIRT1 brain gene in EX-527+LPS group was relevantly lower than other groups (p<0.05). There was a considerable rise in SIRT1 gene expression of Res+LPS group in comparison with K group (p<0.05). Histopathologically, brain sections of K and Res groups had normal morphological characterictics and neurons in LPS group showed degenerative alterations as there were a few degenaration in Res+LPS groups. Degenerative neuron density in Res+LPS group decreased compared to LPS group prominently and neuron degeneration was more elevated in EX-527+LPS group than K and Res groups (p<0.05), and had more less neuron degeneration density than LPS group (p<0.05). Conclusion: It has been concluded that LPS-induced neurodegeration can be prevented by enhancing SIRT1 gene expression with Resveratrol. Key Words: SIRT1, neuroinflammation, neurodegeneration, resveratrol, LPS, EX-527, rat
Aim: The aim of this study is investigating the effects of resveratrol and EX-527 on LPS-induced neuroinflammation rat model brain as molecular genetic, biochemical, cognitive and histopathological and whether there is any neuroprotective activity. Material and Method: This study was carried out with 55 Wistar albino male rats. SIRT1 gene expression, biochemichal analyses and histopathological examination were made in rat brain tissue. Analysis of cognitive effects of disease model was practiced by Morris water maze. Results: In this study, MDA levels of brain tissues in Res and Res+LPS groups were found statistically higher than K, LPS and EX-527+LPS groups (p<0.05). SOD activities in brain tissues of EX-527+LPS were increased in comparision with K and LPS groups statistically (p<0.05) while decreased in Res and Res+LPS groups was significantly compared to K group (p<0.05). Expression of SIRT1 brain gene in EX-527+LPS group was relevantly lower than other groups (p<0.05). There was a considerable rise in SIRT1 gene expression of Res+LPS group in comparison with K group (p<0.05). Histopathologically, brain sections of K and Res groups had normal morphological characterictics and neurons in LPS group showed degenerative alterations as there were a few degenaration in Res+LPS groups. Degenerative neuron density in Res+LPS group decreased compared to LPS group prominently and neuron degeneration was more elevated in EX-527+LPS group than K and Res groups (p<0.05), and had more less neuron degeneration density than LPS group (p<0.05). Conclusion: It has been concluded that LPS-induced neurodegeration can be prevented by enhancing SIRT1 gene expression with Resveratrol. Key Words: SIRT1, neuroinflammation, neurodegeneration, resveratrol, LPS, EX-527, rat
Açıklama
Anahtar Kelimeler
Genetik, Genetics, Nöroloji, Neurology, Tıbbi Biyoloji, Medical Biology
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Tunç, S. (2019). Beyin nöroinflamasyon modelinde resveratrolün oksidatif ve histopatolojik değişiklikler ile SIRT1 geni ifadesi üzerine etkileri. Yayınlanmış Yüksek Lisans Tezi, 1-95 s. İnönü Üniversitesi Malatya.
