Intracerebroventricular injection of spexin stimulates the hypothalamic-pituitary-testicular axis and increases the secretion of male reproductive hormones in rats

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dc.authoridYASIN, Rida zahiraldin ibrahim/0009-0005-6683-1186
dc.contributor.authorYilmaz, Nesibe
dc.contributor.authorIbrahim, Rida Zahiraldin
dc.contributor.authorYildiz, Azibe
dc.date.accessioned2024-08-04T20:56:12Z
dc.date.available2024-08-04T20:56:12Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Male reproductive functions are regulated in the hypothalamic-pituitary-gonadal (HPG) axis. Any problem in this axis would lead to the deterioration of reproductive functions. The present study aimed to investigate the effects of intracerebroventricular (icv) Spexin (SPX) infusion on the HPG axis in detail. Methods: 40 Wistar albino rats were divided into four groups: control, sham, SPX 30 nmol and SPX 100 nmol (n=10). 30 nmol/1 mu l/hour SPX was administered icv to the rats in the SPX 30 nmol group for 7 days, while rats in the SPX 100 nmol group were administered 100 nmol/1 mu l/hour SPX. On the 7th day, the rats were decapitated, blood and tissue samples were collected. Serum LH, FSH and testosterone levels were determined with the ELISA method, GnRH mRNA expression level was determined in hypothalamus with the RT-PCR method. Seminiferous tubule diameter and epithelial thickness were determined with the hematoxylin-eosin staining method. Results: SPX infusion was increased GnRH mRNA expression in the hypothalamus tissue independent of the dose (p<0.05). Serum LH, FSH and testosterone levels in the SPX groups were increased when compared to the control and sham groups independent of the dose (p <0.05). Histological analysis revealed that SPX infusion did not lead to any changes in seminiferous epithelial thickness, while the tubule diameter increased in the SPX groups (p<0.05). Conclusion: The study findings demonstrated that icv SPX infusion stimulated the HPG axis and increased the secretion of male reproductive hormones.en_US
dc.description.sponsorshipKarabuk University Scientific Research Projects Unit [KBUBAP-23-YL004]en_US
dc.description.sponsorshipThis study was carried out with the project number KBUBAP-23-YL004 of Karabuk University Scientific Research Projects Unit.en_US
dc.identifier.doi10.1016/j.aanat.2024.152300
dc.identifier.issn0940-9602
dc.identifier.issn1618-0402
dc.identifier.pmid38971451en_US
dc.identifier.scopus2-s2.0-85197653078en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.aanat.2024.152300
dc.identifier.urihttps://hdl.handle.net/11616/102098
dc.identifier.volume255en_US
dc.identifier.wosWOS:001267331200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofAnnals of Anatomy-Anatomischer Anzeigeren_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIntracerebroventicular (icv)en_US
dc.subjectSpexinen_US
dc.subjectFSHen_US
dc.subjectLHen_US
dc.subjectTestosteroneen_US
dc.subjectGnRHen_US
dc.titleIntracerebroventricular injection of spexin stimulates the hypothalamic-pituitary-testicular axis and increases the secretion of male reproductive hormones in ratsen_US
dc.typeArticleen_US

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