In vitro effect of carbonic anhydrase inhibitor acetazolamide on cell viability, migration and colony formation of colorectal cancer cells

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dc.authoridÜnüvar, Songül/0000-0001-8454-490X
dc.authoridKARAKUŞ, Fuat/0000-0002-5260-3650
dc.authorwosidÜnüvar, Songül/ABH-5516-2020
dc.authorwosidKARAKUŞ, Fuat/O-2627-2019
dc.contributor.authorKarakus, Fuat
dc.contributor.authorEyol, Ergul
dc.contributor.authorYilmaz, Kadir
dc.contributor.authorUnuvar, Songul
dc.date.accessioned2024-08-04T20:44:34Z
dc.date.available2024-08-04T20:44:34Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAcidification of extracellular medium in malignant tumors increases the invasive behaviors of cancer cells. In normal healthy tissues, acid production is catalyzed by carbonic anhydrases. Some of the carbonic anhydrase enzymes are overexpressed in certain types of cancer. The present study aimed to investigate the effect of acetazolamide, a potent carbonic anhydrase inhibitor, on in vitro cultivated cancer cells. Three different assays (MTT test, wound healing and clonogenic assay) were performed using human colorectal adenocarcinoma cells (SW620) to evaluate the suppressive effect of acetazolamide, on the colorectal cancer cells migration ability, colony formation and cell viability. The dose-dependent (1-1000 mu M) reducing effect of acetazolamide on the cell viability was more significant within the first 48 h. This inhibitory effect of acetazolamide was found to be decreased at 72 h, and affects cells migration ability of cells at 24 and 48 h. Acetazolamide was observed to inhibit the cell viability, migration and colony formation ability of cells, depending on dose.en_US
dc.description.sponsorshipInonu University Scientific Research Projects Coordination Unit [2014/29]en_US
dc.description.sponsorshipThe study was supported by Inonu University Scientific Research Projects Coordination Unit with project number 2014/29. The authors would like to thank Prof. Dr. Martin R. Berger (The German Cancer Research Center, Heidelberg, Germany). We thank the anonymous Reviewers for their valuable comments.en_US
dc.identifier.doi10.2478/s11756-018-0064-z
dc.identifier.endpage628en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85047979153en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage621en_US
dc.identifier.urihttps://doi.org/10.2478/s11756-018-0064-z
dc.identifier.urihttps://hdl.handle.net/11616/98315
dc.identifier.volume73en_US
dc.identifier.wosWOS:000441013700010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectColorectal canceren_US
dc.subjectAcetazolamideen_US
dc.subjectCarbonic anhydraseen_US
dc.subjectSW620en_US
dc.subjectAquaporinsen_US
dc.titleIn vitro effect of carbonic anhydrase inhibitor acetazolamide on cell viability, migration and colony formation of colorectal cancer cellsen_US
dc.typeArticleen_US

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