A comparative study of biomarkers of oxidative DNA damage used to detect free radical damage in tissue-engineered skin

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dc.authorscopusid57202786504
dc.authorscopusid7003834140
dc.authorscopusid6602782865
dc.authorscopusid7402080469
dc.authorscopusid7006776689
dc.authorscopusid35591877500
dc.contributor.authorRodriguez H.
dc.contributor.authorJaruga P.
dc.contributor.authorBirincioglu M.
dc.contributor.authorBarker P.E.
dc.contributor.authorO'Connell C.
dc.contributor.authorDizdaroglu M.
dc.date.accessioned2024-08-04T19:59:14Z
dc.date.available2024-08-04T19:59:14Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe process of tissue engineering often involves the mixing of cells with polymers that may elevate the level of endogenous free radicals and thus cause genetic damage to the cell. In order to assure that such composite materials are free of genetic damage, our laboratory is responding to the need for test methods used to assess the safety and performance of tissue-engineered materials. Specifically, we are identifying cellular biomarkers that could be used to ensure that the cells of tissue-engineered materials have not undergone any oxidative DNA damage from the production of free radicals by oxidative stress during the development, storage or shipment of the product. Using the technique of gas chromatography/mass spectrometry, we have screened for the oxidatively modified DNA base 8-hydroxyguanine in tissue-engineered skin and compared the levels to those in control cells, neonatal fibroblasts and neonatal keratinocytes. No significant level of damage was detected compared to control cells. The technique of liquid chromatography/mass spectrometry was also used in the validation of this biomarker by measuring its nucleoside form. The results obtained with this technique were nearly identical to those obtained with gas chromatography/mass spectrometry. Biomarker programs such as this can provide the basis for an international reference standard of cellular biomarkers that can aid in the development and safety of tissue-engineered medical products.en_US
dc.identifier.doi10.1520/stp11638s
dc.identifier.endpage89en_US
dc.identifier.issn0066-0558
dc.identifier.issue1452en_US
dc.identifier.scopus2-s2.0-3242661829en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage84en_US
dc.identifier.urihttps://doi.org/10.1520/stp11638s
dc.identifier.urihttps://hdl.handle.net/11616/90486
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherASTM Internationalen_US
dc.relation.ispartofASTM Special Technical Publicationen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiomarkersen_US
dc.subjectFree radical damageen_US
dc.subjectOxidative DNA damageen_US
dc.titleA comparative study of biomarkers of oxidative DNA damage used to detect free radical damage in tissue-engineered skinen_US
dc.typeConference Objecten_US

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