The relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosis

dc.authoridKAYHAN, BASAK/0000-0003-3508-2563
dc.authoridtoplu, sibel altunışık/0000-0002-2915-4666
dc.authoridBayindir, Yasar/0000-0003-3930-774X
dc.authoridYesilada, Elif/0000-0002-3743-5767
dc.authoridKARACA, Zeynal Mete/0000-0002-5967-8102
dc.authoridKayabas, Uner/0000-0002-5323-0796
dc.authorwosidKAYHAN, BASAK/ABH-9314-2020
dc.authorwosidtoplu, sibel altunışık/ABH-5816-2020
dc.authorwosidKARACA, Zeynal Mete/O-1138-2018
dc.authorwosidBayindir, Yasar/T-1523-2017
dc.authorwosidYesilada, Elif/ABG-9600-2020
dc.authorwosidKARACA, Zeynal Mete/HKM-7924-2023
dc.authorwosidKayabas, Uner/JRX-1616-2023
dc.contributor.authorKaraca, Gamze
dc.contributor.authorKaraca, Zeynal Mete
dc.contributor.authorKayhan, Basak
dc.contributor.authorBayindir, Yasar
dc.contributor.authorKayabas, Uner
dc.contributor.authorToplu, Sibel
dc.contributor.authorElmasdag, Sirvan
dc.date.accessioned2024-08-04T20:10:31Z
dc.date.available2024-08-04T20:10:31Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractOBJECTIVE: Brucellosis is a zoonotic disease caused by Brucella in domestic and wild animals. It also causes systemic diseases with the involvement of different parts of the human body. An efficient innate immune response is crucial to cure brucellosis with optimum antibiotic treatment. The inflammasomes are innate immune system receptors and sensors that regulate the activation of cysteine-dependent aspartate specific protease-1 (caspase-1) and caspase-1-induced cell death process known as pyroptosis. The aim of the present study was to investigate the expression levels of CASPASE-1 and associated inflammasomes AIM2, NLRP3, and NLRC4 to analyze their relationship with the inflammatory cytokine interleukin (IL)-1 beta, IL-18, and interferon-gamma (IFN-gamma) in peripheral blood samples of patients with acute brucellosis with healthy controls. METHODS: Peripheral blood samples were obtained from 20 healthy volunteers and 20 patients with acute brucellosis. RNA and serum samples were isolated to examine the expression levels of AIM2, NLRP3, NLRC4, and CASPASE-1 by real-time polymerase chain reaction, and IL-1 beta, IL-18, and IFN-gamma were measured by enzyme-linked immunosorbent assay. RESULTS: In the acute brucellosis group, AIM2 and NLRC4 expressions were significantly higher than in healthy volunteers. A significant increase on caspase-1 expression in patients with acute brucellosis was not observed. Serum IL-18 and IFN-gamma levels were significantly higher in patients with acute brucellosis than in healthy controls. CONCLUSION: Caspase-1-related inflammasomes are sufficiently activated to induce the secretion of cytokines, such as IFN-gamma and IL-18, to induce cellular immune response. Caspase-1 activation level should be investigated at different periods of disease in a group with high number of patients to understand the role of pyroptosis and caspase-1 in brucellosis.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Inonu University [2013/77]en_US
dc.description.sponsorshipThis study has been supported by the Scientific Research Projects Coordination Unit of Inonu University (project no. 2013/77).en_US
dc.identifier.doi10.14744/nci.2018.96992
dc.identifier.endpage123en_US
dc.identifier.issn2148-4902
dc.identifier.issue2en_US
dc.identifier.pmid31297476en_US
dc.identifier.startpage117en_US
dc.identifier.trdizinid337788en_US
dc.identifier.urihttps://doi.org/10.14744/nci.2018.96992
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/337788
dc.identifier.urihttps://hdl.handle.net/11616/92824
dc.identifier.volume6en_US
dc.identifier.wosWOS:000472063600003en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKare Publen_US
dc.relation.ispartofNorthern Clinics of Istanbulen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAcute brucellosisen_US
dc.subjectcaspase-1en_US
dc.subjectcytokinesen_US
dc.subjectinflammasomesen_US
dc.titleThe relationship between caspase-1 related inflammasome expression and serum inflammatory cytokine levels during acute brucellosisen_US
dc.typeArticleen_US

Dosyalar