Vitreoscilla hemoglobin expressing Enterobacter aerogenes and Pseudomonas aeruginosa respond differently to carbon catabolite and oxygen repression for production of L-asparaginase, an enzyme used in cancer therapy

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dc.authoridUÇKUN, Miraç/0000-0002-9018-8515
dc.authoridGeckil, Hikmet/0000-0003-0070-0691;
dc.authorwosidUÇKUN, Miraç/ABC-4209-2021
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.authorwosidMiraç, Uçkun/ABG-5824-2021
dc.contributor.authorGeckil, H
dc.contributor.authorGencer, S
dc.contributor.authorUckun, M
dc.date.accessioned2024-08-04T20:15:13Z
dc.date.available2024-08-04T20:15:13Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe production of antileukemic enzyme L-asparaginase in two distinctly related bacteria, Enterobacter aerogenes, Pseudomonas aeruginosa, and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. Both bacteria showed a substantially different degree of carbon catabolite repression of the enzyme production. E. aerogenes grown under catabolite repression had more than 20-fold lower L-asparaginase activity than the controls. This figure was only 1.6-fold for P. aeruginosa. In the medium with restricted nutrient content, however, the inhibitory effect of glucose on the enzyme production was less pronounced. The presence of VHb, an efficient oxygen uptake system, had also different effects in both bacteria. Under conditions of no catabolite repression, this protein caused about 7-fold lower L-asparaginase activity in E. aerogenes, but similar or even slightly stimulatory effect in P aeruginosa. The use of a relatively poor carbon source, mannitol, caused a lower L-asparaginase level and no glucose type catabolite repression. (C) 2004 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.enzmictec.2004.04.005
dc.identifier.endpage189en_US
dc.identifier.issn0141-0229
dc.identifier.issn1879-0909
dc.identifier.issue2-3en_US
dc.identifier.scopus2-s2.0-3142636524en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage182en_US
dc.identifier.urihttps://doi.org/10.1016/j.enzmictec.2004.04.005
dc.identifier.urihttps://hdl.handle.net/11616/94247
dc.identifier.volume35en_US
dc.identifier.wosWOS:000223032200011en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofEnzyme and Microbial Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.subjectbacterial hemoglobinen_US
dc.subjectL-asparaginaseen_US
dc.subjectchemotherapeutic enzymesen_US
dc.subjectcatabolite repressionen_US
dc.subjectoxygen-regulated genesen_US
dc.titleVitreoscilla hemoglobin expressing Enterobacter aerogenes and Pseudomonas aeruginosa respond differently to carbon catabolite and oxygen repression for production of L-asparaginase, an enzyme used in cancer therapyen_US
dc.typeArticleen_US

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