Electrospun Polycaprolactone-Chitosan Nanofiber Scaffolds for Covalent Immobilization of Xylanase: Structural Characterization and Enzyme Performance
Küçük Resim Yok
Tarih
2026
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Xylanases (XyL) are critical enzymes that release that hydrolyze xylan, a major component of hemicellulose, into xylose and its derivates, and they are vital in diverse industrial fields. However, their use in free form is limited due to instability and lack of reusability. Enzyme immobilization offers a promising approach to overcome these drawbacks. In this study, electrospun polycaprolactone/chitosan (PCL/CHI) nanofibers were fabricated and used as by nanocarriers for covalent immobilization of XyL. A solution containing 10% PCL and 0.5% CHI in acetic acid: formic acid (6:4) was electrospun under optimized conditions (22 kV, 1 mL/h, 18 cm). Fibers were cross-linked with glutaraldehyde, washed thoroughly to remove residual toxicity, and then used for enzyme immobilization. Structural and morphological analyses (SEM, EDX, XRD, and FTIR) confirmed successful fiber formation and enzym binding. The optimum temperature was determined as 50 degrees C for both forms, while the optimum pH was determined as 6 for the free form and 5 for the immobilized form. The activation energy decreased from 21.46 kJ/mol to 1.17 kJ/mol, indicating enhaced catalytic efficiency. Kinetic analysis revealed a reduction in Km (13.01\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\to\:$$\end{document} 4.80 mg/mL), suggesting improved substrate affinity. Furthermore, the decrease in Km value revealed that immobilization enhanced enzyme-substrate interaction, while reusability tests showed that the immobilized enzyme retained 45% of its initial activity after five cycles. The fact that the immobilized form maintained its high catalytic performance in the presence of metal ions highlights the system's potential for adaptation to industrial conditions. In conclusion, this developed platform has been demonstrated to be a promising approach for sustainable and economical solutions in enzyme technologies.
Açıklama
Anahtar Kelimeler
XyL, Enzyme immobilization, Electrospinning, Nanofibers, Biocatalysts
Kaynak
Catalysis Letters
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
156
Sayı
2
